您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Extraction of flavonoids from black mulberry wine residues and their antioxidant and anticancer activity in vitro

文献类型： 外文期刊

作者： Ma, Jian 1 ; Li, Peng 3 ; Ma, Yanhong 1 ; Liang, Liya 4 ; Jia, Feihong 1 ; Wang, Yu 2 ; Yu, Lijun 1 ; Huang, Wuyang 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Agroprod Proc, 50 Zhongling St, Nanjing 210014, Peoples R China

2.Shanxi Agr Univ, Coll Food Sci & Engn, Taigu 030801, Peoples R China

3.Jiangsu Acad Agr Sci, Tech Ctr Publ Testing & Evaluat & Identificat, Nanjing 210014, Peoples R China

4.Tianjin Agr Univ, Coll Agron & Resources & Environm, Tianjin 300384, Peoples R China

5.Work Forestry Adm Stn Kirgiz Autonomous Prefecture, Artush 845350, Peoples R China

关键词： Black mulberry wine residues; Flavonoids; Ultrasound -assisted enzymatic extraction; Antioxidant capacity; Anticancer; Apoptosis

期刊名称：HELIYON （ 影响因子：3.4； 五年影响因子：3.9 ）

ISSN：

年卷期： 2024 年 10 卷 10 期

页码：

收录情况： SCI

摘要： Enhancing the valorization of fruit processing by-products is pivotal for advancing the industry. Black mulberry wine residues, a by-product, contains some bioactive compounds, yet its antioxidant and anticancer potentials remain unverified. In this study, ultrasound-assisted enzymatic extraction was optimized by response surface methodology to obtain the flavonoids extracts from black mulberry wine residues, whose antioxidant capacity and anti-cancer activity in vitro was investigated. The results showed that under the optimal extraction conditions (enzyme ratio at pectinase:cellulose = 2:1, mixed enzyme concentration 0.31 mg/mL, enzymatic hydrolysis temperature 55.35 degrees C, enzymatic hydrolysis time 79.03 min, and ultrasonic time 22.71 min), the extracts from black mulberry wine residues (BMWR-E) reached 5.672 mg/g. At a concentration of 1.2 mg/mL, BMWR-E exhibited strong DPPH and hydroxyl radical scavenging activities. At a concentration of 2.5 mg/mL, BMWR-E showed a strong superoxide anion radical scavenging capacity, with no significant distinction compared to the positive control group (Vitamin C) (p > 0.05). Cell viability assay results showed that BMWR-E was non-toxic to normal BRL-3A cells when applied at concentrations of 0.1-0.3 mg/mL for an incubation period of 24 h, but BMWR-E exhibited the ability to inhibit the proliferation of HepG2 cells. At concentrations of 0.2 mg/mL and above, BMWR-E could induce late apoptosis of HepG2 cells by increasing the protein expression levels of Bax, caspase-3, and caspase-12, reducing the protein expression levels of Bcl-2, inducing cell cycle arrest at G0/G1 phase, thereby inhibiting the proliferation of HepG2 cells. The bioactive properties make BMWR-E possess potential in developing new antioxidants and anti-cancer agents, which would significantly enhance the economic worth of agricultural by-products in product processing. This research can improve the utilization rate of agricultural product processing by-products and protect the environment.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台