Novel ACE inhibitory peptides derived from bighead carp (Aristichthys nobilis) hydrolysates: Screening, inhibition mechanisms and the bioconjugation effect with graphene oxide
文献类型： 外文期刊
作者： Chen, Mengting 1 ; Wang, Lan 1 ; Zheng, Changliang 1 ; Ma, Aimin 2 ; Hu, Kai 2 ; Xiang, Aoli 2 ; Sun, Zhida 2 ; Xie, Bijun 2 ; Xiong, Guangquan 1 ; Shi, Liu 1 ; Chen, Sheng 1 ; Wu, Wenjin 1 ;
作者机构： 1.Hubei Acad Agr Sci, Inst Agroprod Proc & Nucl Agr Technol, Minist Agr & Rural Affairs, Key Lab Agr Prod Cold Chain Logist, Wuhan 430064, Hubei, Peoples R China
2.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Hubei, Peoples R China
关键词： ACE inhibitory activity; Bioactive peptides; Molecular docking; Graphene oxide; Bioconjugation
期刊名称：FOOD BIOSCIENCE （ 影响因子：5.2； 五年影响因子：5.4 ）
ISSN： 2212-4292
年卷期： 2023 年 52 卷
页码：
收录情况： SCI
摘要： The ACE inhibitory peptides have been characterized from the enzymatic digestion product of bighead carp protein and bioconjugated with graphene oxide (GO) to enhance its activity. The results showed that aspartic acid and glutamic acid had the highest levels in ultrafiltration fractions (<5 kDa), where eight potential ACE inhibitory peptides were also identified (ADSNHKAF, KLWHHTF, LLRLHF, PPSEPTKL, VEKFPLF, YLRLHF, YYKLKPLL, YYKLKPML). Among the eight peptides, YLRLHF showed the best ACE inhibitory activity (IC50 = 121.90 mu M) and was a competitive inhibitor. Molecular docking experiments showed that YLRLHF formed four hydrogen bond interactions in the ACE protein pocket, coordination bonding with Zn2+, and 7C-7C conjugation interactions to His421. GO elevated the ACE inhibitory activity of YLRLHF (at 0.1 mg/mL) from 43.36% to 51.72%. The structural characterization results obtained from FI-IR, XPS, SEM, and TEM demonstrated the successful combination of GO and YLRLHF. Additionally, biocouples of ACE inhibitory peptides from bighead carp proteins with GO might be potential candidates for future functional foods and antihypertensive drugs.
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