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Integration analysis of PacBio SMRT- and Illumina RNA-seq reveals P450 genes involved in thiamethoxam detoxification in Bradysia odoriphaga

文献类型： 外文期刊

作者： Chen, Chengyu 1 ; Wang, Cuicui 2 ; Liu, Ying 3 ; Shan, Tisheng 2 ; Shi, Xueyan 2 ; Gao, Xiwu 2 ;

作者机构： 1.Jiangsu Acad Agr Sci, Huaiyin Inst Agr Sci Xuhuai Reg Jiangsu, Huaian 223001, Jiangsu, Peoples R China

2.China Agr Univ, Coll Plant Protect, Dept Entomol, Beijing 100193, Peoples R China

3.Yunnan Acad Agr Sci, Inst Agr Resources & Environm, Key Lab Green Prevent & Control Agr Transboundary, Kunming 650205, Peoples R China

4.2 West YuanMingyuan Rd, Beijing, Peoples R China

关键词： Bradysia odoriphaga; SMRT sequencing; Illumina sequencing; P450s; Thiamethoxam detoxification

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.966； 五年影响因子：4.591 ）

ISSN： 0048-3575

年卷期： 2022 年 186 卷

页码：

收录情况： SCI

摘要： The sciarid fly Bradysia odoriphaga is a serious pest of Chinese chive (Liliaceae). Neonicotinoid insecticides including thiamethoxam have been used for B. odoriphaga control. However, thiamethoxam resistance in B. odoriphaga has developed in recent years. To identify potential genes involved in detoxification metabolism of thiamethoxam in B. odoriphaga, a PacBio single-molecule real-time (SMRT) transcriptome sequencing and Illumina RNA-seq analysis on thiamethoxam treated B. odoriphaga were performed to explore differentially expressed genes in B. odoriphaga. After SMRT sequencing, analysis of Illumina RNA-Seq data showed a total of 172 differentially expressed genes (DEGs) after thiamethoxam treatment, among which eight upregulated DEGs were P450 genes that may be related to thiamethoxam metabolism. The qRT-PCR results of the eight upregulated P450 unigenes after thiamethoxam treatment were consistent with RNA-Seq data. Furthermore, oral delivery mediated RNA interference of the eight upregulated P450 transcripts followed by insecticide bioassay was conducted, and three P450 unigenes were verified to be related to thiamethoxam detoxification in B. odoriphaga. This study provides new information about the P450 genes involved in thiamethoxam detoxification in B. odoriphaga.

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