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Purification, identification and hypolipidemic activities of three novel hypolipidemic peptides from tea protein

文献类型： 外文期刊

作者： Ye, Haoduo 1 ; Xu, Yan 2 ; Sun, Yunnan 3 ; Liu, Benying 3 ; Chen, Bingbing 1 ; Liu, Guo 1 ; Cao, Yong 1 ; Miao, Jianyin 1 ;

作者机构： 1.South China Agr Univ, Coll Food Sci, Guangdong Prov Key Lab Nutraceut & Funct Foods, Guangzhou 510642, Peoples R China

2.Anhui Agr Univ, State Key Lab Tea Plant Biol & Utilizat, 130 Changjiang West Rd, Hefei 230036, Anhui, Peoples R China

3.Yunnan Acad Agr Sci, Tea Res Inst, Yunnan Prov Key Lab Tea Sci, Menghai 666201, Peoples R China

4.Guangdong Acad Agr Sci, Tea Res Inst, Guangdong Prov Key Lab Tea Plant Resources Innovat, Guangzhou 510640, Peoples R China

5.Huanggang Normal Univ, Hubei Collaborat Innovat Ctr Characterist Resource, Hubei Key Lab Econ Forest Germplasm Improvement &, Huanggang 438000, Peoples R China

6.South China Agr Univ, Coll Food Sci, Guangzhou 510642, Peoples R China

关键词： Tea protein; Purification; Identification; Molecular docking; Hypolipidemic peptides; Hypolipidemic activity

期刊名称：FOOD RESEARCH INTERNATIONAL （ 影响因子：8.1； 五年影响因子：7.7 ）

ISSN： 0963-9969

年卷期： 2023 年 165 卷

页码：

收录情况： SCI

摘要： In this study, hypolipidemic peptides were obtained from tea protein by enzymatic hydrolysis, ultrafiltration and high-performance liquid chromatography. Subsequently, the hypolipidemic peptides were identified by mass spectrometry and screened through molecular docking technology, and the hypolipidemic activities and mechanisms of the active peptides were explored. The results showed that the hydrolysate of hypolipidemic peptides obtained by pepsin hydrolysis for 3 h had good bile salt binding ability. After purification, identification and molecular docking screening, three novel hypolipidemic peptides FLF, IYF and QIF were obtained. FLF, IYF and QIF can interact with the receptor proteins 1LPB and 1F6W through hydrogen bonds, 7C-7C bonds, hydrophobic interactions and van der Waals forces, thus exerting hypolipidemic activities. Activity studies showed that, compared with the positive controls, FLF, IYF and QIF had excellent sodium taurocholate binding abilities, pancreatic lipase inhibitory activities and cholesterol esterase inhibitory activities. Moreover, FLF, IYF and QIF can effectively inhibit lipogenic differentiation of 3T3-L1 preadipocytes, reduce intracellular lipid and lowdensity lipoprotein content and increase high-density lipoprotein content. These results indicated that the three novel hypolipidemic peptides screened in this study had excellent hypolipidemic activities and were expected to be used as natural-derived hypolipidemic active ingredients for the development and application in functional foods.

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