Identification and functional analysis of two potential RNAi targets for chitin degradation in Holotrichia parallela Motschulsky (Insecta Coleoptera)
文献类型： 外文期刊
作者： Zhao, Dan 1 ; Liu, Xiaomin 2 ; Liu, Zhaorui 1 ; Wu, Han 1 ; Lu, Xiujun 1 ; Guo, Wei 1 ;
作者机构： 1.Hebei Agr Univ, Coll Plant Protect, Baoding, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Key Lab Crop Cultivat Physiol & Green Prod Hebei P, Shijiazhuang, Peoples R China
3.Chinese Acad Agr Sci, Grad Sch, Beijing, Peoples R China
关键词： Holotrichia parallela; NAG and NAGK; RNA interference; Chitin
期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.966； 五年影响因子：4.591 ）
ISSN： 0048-3575
年卷期： 2022 年 188 卷
页码：
收录情况： SCI
摘要： Chitin metabolism enzymes are safe and desirable targets for pest management. beta-N-acetylglucosaminidase (NAG) and N-acetylglucosamine kinase (NAGK) are involved in chitin degradation. NAG is the main glycosidase that works synergistically with chitinases. NAGK is a key enzyme for the generation of UDP-Nacetylglucosamine (UDP-GlcNAc) and for the conversion of GlcNAc into GlcNAc 6-phosphate (GlcNAc-6-P). In this study, NAG and NAGK genes were identified from Holotrichia parallela, a polyphagous soil pest that causes serious damage to crops. The spatiotemporal expression investigated by RT-qPCR indicated that the two genes are expressed in all larval developmental stages. HpNAG is highly expressed in the integument and HpNAGK overexpressed in the midgut. After injection of dsHpNAG and dsHpNAGK, a significant RNAi effect was found after 72 h and larvae stopped growing. The survival rates of larvae were 13.3% and 16.7%, respectively. RNAi of HpNAG and HpNAGK regulated the expression levels of chitin metabolism-related genes, indicating that these two genes could be critical in the chitin metabolism. Furthermore, silencing HpNAG and HpNAGK reduced the thickness of the cuticle, and decreased its content of chitin. The study will lay a foundation for further clarifying the mechanism of chitin metabolism and provide potential targets for the biological control of H. parallela larvae.
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