文献类型： 外文期刊

作者： Tang, Guirong ¹ ; Song, Shuangxiu ¹ ; Shang, Junmei ¹ ; Luo, Yujuan ¹ ; Li, Shiqin ¹ ; Wei, Dongxiang ¹ ; Wang, Chengshu ¹ ;

作者机构： 1.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, Shanghai Inst Plant Physiol & Ecol, Key Lab Insect Dev & Evolutionary Biol, Shanghai 200032, Peoples R China

2.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201106, Peoples R China

3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China

4.Univ Chinese Acad Sci, CAS Ctr Excellence Biot Interact, Beijing 100049, Peoples R China

关键词： Metarhizium; effector; antifungal immunity; cathepsin |; Persephone

期刊名称：PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA （ 影响因子：9.1； 五年影响因子：10.6 ）

ISSN： 0027-8424

年卷期： 2025 年 122 卷 3 期

页码：

收录情况： SCI

摘要： Entomopathogenic fungi play a critical role in regulating insect populations, and representative species from the Metarhizium and Beauveria genera have been developed as eco- friendly biocontrol agents for managing agricultural insect pests. Relative to the advances in understanding antifungal immune responses in Drosophila, knowledge of how fungi evade insect immune defenses remains limited. In this study, we report the identification and characterization of a virulence- required effector Fkp1 in Metarhizium robertsii. Library screening and protein pull- down analysis unveiled that Fkp1 targets the cathepsin protease CtsK1 to inhibit its cleavage maturation of the danger- sensing serine protease Persephone (Psh), thereby facilitating fungal evasion of the Drosophila immune defenses. The Fkp1-like gene is also required in Beauveria bassiana for insect infection. Transgenic expression of Fkp1 in Drosophila suppressed hemolymph cysteine protease activity and down- regulated the expression of antifungal genes. Fkp1 can also mask the Psh cleavage site without interfering with its ability to bait fungal subtilisin proteases. Given the evident compensatory relationship, our data indicate that the protease cascade is more crucial than the molecular pattern pathway in defending flies against fungal infections. This work reveals that Metarhizium fungi have evolved distinct effectors to block the dual recognition pathways of flies for immune evasion and sheds lights on the effector mechanisms mediating microbe-animal interactions.