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Positively Charged-Amylose-Entangled Au-Nanoparticles Acting as Protein Carriers and Potential Adjuvants to SARS-CoV-2 Subunit Vaccines

文献类型： 外文期刊

作者： Fan, Baochao 1 ; Gu, Jun 1 ; Deng, Bin 1 ; Guo, Weilu 1 ; Zhang, Shuaifeng 1 ; Li, Li 1 ; Li, Bin 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Minist Sci & Technol, Inst Vet Med, Minist Agr,Key Lab Vet Biol Engn & Technol,Jiangsu, Nanjing 210014, Peoples R China

2.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225000, Jiangsu, Peoples R China

3.GuoTai Taizhou Ctr Technol Innovat Vet Biol, Taizhou 225300, Peoples R China

4.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212000, Peoples R China

5.China Pharmaceut Univ, Coll Pharm, Nanjing 210000, Peoples R China

6.Nanjing Tech Univ, Sch Pharmaceut, Nanjing 210000, Peoples R China

7.Jiangsu Univ, Sch Mat Sci & Engn, Zhenjiang 212000, Peoples R China

关键词： antigen carrier; amylose; Au nanoparticle; chitosan derivative; coronavirus subunit vaccine

期刊名称：ACS APPLIED MATERIALS & INTERFACES （ 影响因子：9.5； 五年影响因子：9.6 ）

ISSN： 1944-8244

年卷期： 2023 年 15 卷 25 期

页码：

收录情况： SCI

摘要： TheCOVID-19 pandemic continues to spread worldwide. To protectand control the spread of SARS-CoV-2, varieties of subunit vaccinesbased on spike (S) proteins have been approved for human applications.Here, we report a new subunit vaccine design strategy that functionsas both an antigen carrier and an adjuvant in immunization to elicithigh-level immune responses. The complex of 2-hydroxypropyl-trimethylammoniumchloride chitosan and amylose entangles Au nanoparticles (HTCC/amylose/AuNPs)forming 40 nm nanocarriers with a positive charge. The obtained positivelycharged nanoparticles reveal many merits, including the larger S proteinloading capacity in PBS buffer, higher cellular uptake ability, andlower cell cytotoxicity, supporting their potential as safe vaccinenanocarriers. Two functionalized nanoparticle subunit vaccines areprepared via loading full-length S proteins derived from SARS-CoV-2variants. In mice, both prepared vaccines elicit high specific IgGantibodies, neutralize antibodies, and immunoglobulin IgG1 and IgG2a.The prepared vaccines also elicit robust T- and B-cell immune responsesand increase CD19(+) B cells, CD11C(+) dendriticcells, and CD11B(+) macrophages at the alveoli and bronchiof the immunized mice. Furthermore, the results of skin safety testsand histological observation of organs indicated in vivo safety ofHTCC/amylose/AuNP-based vaccines. Summarily, our prepared HTCC/amylose/AuNPhave significant potential as general vaccine carriers for the deliveryof different antigens with potent immune stimulation.

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