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Splicing factor Nova regulates the splice variants in exons 3 and 6 of GABA receptor subunit RDL from Chilo suppressalis Walker (Lepidoptera: Crambidae)

文献类型： 外文期刊

作者： Song, Zijiao 1 ; Jiang, Jie 1 ; Tang, Tao 2 ; Luo, Guanghua 3 ; Wang, Junyan 1 ; Lin, Kaixiang 1 ; Mu, Lili 1 ; Zhao, Chunqing 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, State Key Lab Agr & Forestry Biosecur, Nanjing 211800, Peoples R China

2.Hunan Acad Agr Sci, Inst Plant Protect, Changsha 410125, Peoples R China

3.Jiangsu Acad Agr Sci, Inst Plant Protect, Nanjing 210014, Peoples R China

关键词： Exon 3; Neuron system; Nova; Trans-acting splicing factor; RNA interference; Molecular docking

期刊名称：PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY （ 影响因子：4.0； 五年影响因子：4.5 ）

ISSN： 0048-3575

年卷期： 2025 年 214 卷

页码：

收录情况： SCI

摘要： The insect ionotropic gamma-aminobutyric acid (GABA) receptor is an important insecticide target, and alternative splicing (AS) among exons 3a, 3b, 6a, and 6b of its RDL subunit is ubiquitous in insects; however, the AS factors and mechanisms remain unclear. While the neuro-oncological ventral antigen (Nova) is known to regulate AS of the gamma 2 subunit of mammalian GABAA receptors, its role in insects remains unexplored. Two CsNova isoforms, CsNova-X1 and CsNova-X3, were identified by BLAST in the third-generation transcriptome of Chilo suppressalis. These isoforms exhibited conserved KH domains with the open reading frames of CsNova-X1 and CsNova-X3 measuring 1638 bp and 1572 bp, respectively. Compared with CsNova-X1, CsNova-X3 lacks 22 amino acid residues in the N-terminal region. Developmental expression profiling revealed peak mRNA levels during the pupal stage. RNA interference (RNAi)-mediated knockdown of CsNova significantly reduced CsRDL-3a/3b/6a transcript levels after 72 h. Molecular docking simulations revealed that the UCAUCAC motif in the downstream of exon 2 of CsRDL could interact with CsNova-X1, while the UCAU and UCAC motifs in exon 6b and its flanking regions could interact with CsNova-X3. In conclusion, Nova was cloned from the Lepidoptera insect for the first time and could regulate the AS process of exon 3 and exon 6 of CsRDL, laying a foundation for in-depth studies on the regulatory mechanism of AS for RDL and the development of RNAi-based pesticides targeting these interactions.

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