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Efficient breeding of low glutelin content rice germplasm by simultaneous editing multiple glutelin genes via CRISPR/Cas9

文献类型： 外文期刊

作者： Chen, Zhihui 1 ; Du, Hongxu 3 ; Tao, Yajun 1 ; Xu, Yang 1 ; Wang, Fangquan 1 ; Li, Bin 1 ; Zhu, Qian-Hao 5 ; Niu, Hongbin 3 ; Yang, Jie 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Chinese Natl Ctr Rice Improvement, Nanjing Branch, Inst Food Crops, Nanjing 210014, Jiangsu, Peoples R China

2.Yangzhou Univ, Jiangsu Coinnovat Ctr Modern Prod Technol Grain C, Yangzhou 225009, Jiangsu, Peoples R China

3.Henan Agr Univ, Coll Agron, Zhengzhou 450002, Henan, Peoples R China

4.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Nanjing, Peoples R China

5.CSIRO Agr & Food, GPO Box 1700, Canberra, ACT 2601, Australia

关键词： Rice; CRISPR/Cas9; Gene editing; Glutelin content; Grain quality; Breeding

期刊名称：PLANT SCIENCE （ 影响因子：5.363； 五年影响因子：5.454 ）

ISSN： 0168-9452

年卷期： 2022 年 324 卷

页码：

收录情况： SCI

摘要： Chronic kidney disease (CKD) and phenylketonuria (PKU) patients need to eat rice with low glutelin content. Therefore, breeding low glutelin content rice varieties with high yield and delicious taste is one of the major goals of rice breeders due to the high demand for the product. In this study, we designed three sgRNAs targeting nine glutelin genes and generated nine T-DNA-free homozygous editing lines with reduced glutelin content compared with the wild-type due to simultaneous mutation(s) in 5-7 glutelin genes. The glutelin content of two lines is even significantly lower than that of the low glutelin content cultivar, LGC-1. Compared to the wild-type, these low glutelin lines showed similar agronomic traits, including yield components and viscosity properties, and can be used as new varieties or parental materials for further breeding.

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