文献类型： 外文期刊

作者： Jia, Ding-Jia-Cheng ¹ ; Wang, Qi-Wen ² ; Hu, Ying-Ying ² ; He, Jia-Min ² ; Ge, Qi-Wei ¹ ; Qi, Ya-Dong ² ; Chen, Lu-Yi ² ; Zhang, Ying ² ; Fan, Li-Na ¹ ; Lin, Yi-Feng ¹ ; Sun, Yong ¹ ; Jiang, Yao ¹ ; Wang, Lan ² ; Fang, Yan-Fei ² ; He, Hui-Qin ³ ; Pi, Xiong-E ⁵ ; Liu, Wei ⁵ ; Chen, Shu-Jie ² ; Wang, Liang-Jing ¹ ;

作者机构： 1.Zhejiang Univ, Affiliated Hosp 2, Sch Med, Dept Gastroenterol, Hangzhou, Peoples R China

2.Zhejiang Univ, Inst Gastroenterol, Hangzhou, Peoples R China

3.Zhejiang Univ, Sir Run Run Shaw Hosp, Dept Gastroenterol, Hangzhou, Peoples R China

4.Zhejiang Univ, Sir Run Run Shaw Hosp, Dept Gen Practice, Hangzhou 310058, Peoples R China

5.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Hangzhou, Peoples R China

6.Zhejiang Univ, Canc Ctr, Hangzhou, Peoples R China

7.Zhejiang Univ, Affiliated Hosp 2, Sch Med, Hangzhou 310009, Zhejiang, Peoples R China

8.Zhejiang Univ, Sir Run Run Shaw Hosp, Hangzhou 310003, Zhejiang, Peoples R China

9.Zhejiang Acad Agr Sci, Hangzhou 310021, Zhejiang, Peoples R China

关键词： Lactobacillus johnsonii; Ulcerative colitis; Macrophages; STAT3; IL-10

期刊名称：GUT MICROBES （ 影响因子：9.434； 五年影响因子：11.724 ）

ISSN： 1949-0976

年卷期： 2022 年 14 卷 1 期

页码：

收录情况： SCI

摘要： Imbalance of gut microbiota homeostasis is related to the occurrence of ulcerative colitis (UC), and probiotics are thought to modulate immune microenvironment and repair barrier function. Here, in order to reveal the interaction between UC and gut microbiota, we screened a new probiotic strain by 16S rRNA sequencing from Dextran Sulfate Sodium (DSS)-induced colitis mice, and explored the mechanism and clinical relevance. Lactobacillus johnsonii (L. johnsonii), as a potential anti-inflammatory bacterium was decreased colonization in colitis mice. Gavage L. johnsonii could alleviate colitis by specifically increasing the proportion of intestinal macrophages and the secretion of Il-10 with macrophages depleted model and in Il10(-/-) mice. We identified this subset of immune cells activated by L. johnsonii as CD206(+) macrophages(IL-10). Mechanistically, L. johnsonii supplementation enhanced the mobilization of CD206(+) macrophages(IL-10) through the activation of STAT3 in vivo and in vitro. In addition, we revealed that TLR1/2 was essential for the activation of STAT3 and the recognition of L. johnsonii by macrophages. Clinically, there was positive correlation between the abundance of L. johnsonii and the expression level of MRC1, IL10 and TLR1/2 in UC tissues. L. johnsonii could activate native macrophages into CD206(+) macrophages and release IL-10 through TLR1/2-STAT3 pathway to relieve experimental colitis. L. johnsonii may serve as an immunomodulator and anti-inflammatory therapeutic target for UC.