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TYMV induced gene silencing of TrxG family gene BrULT2 affects the leaf shape of chinese cabbage

文献类型： 外文期刊

作者： Li, Rui 1 ; Song, Lixiao 2 ; Yu, Jing 1 ; Hou, Xilin 1 ; Zhang, Changwei 1 ;

作者机构： 1.Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement, Coll Hort, Nanjing 210095, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210014, Jiangsu, Peoples R China

关键词： BRULT1; BRULT2; CHINESE CABBAGE; LEAF SHAPE; VIGS

期刊名称：BIOSCIENCE BIOTECHNOLOGY RESEARCH COMMUNICATIONS

ISSN： 0974-6455

年卷期： 2019 年 12 卷 2 期

页码：

收录情况： SCI

摘要： The SAND domain protein ULTRAPETALA1 (ULT1) and its paralog ULT2 define a small family of closely related plant proteins, both of them physically associated to form heterodimers of trxG (Trithorax group) factors. Although trxG gene ULT1 has well-defined roles in controlling gynoecium patterning and cell fate decisions of plants, relatively little is known about the specific functions of ULT2. In this study, bioinformation analysis revealed that the BrULT2 gene of Brassica rapa encodes a protein of molecular mass of 26.37 KDa, an isoelectric point of 7.9. qRT-PCR was performed to detect the expression profiles of the BrULT2 gene of Chinese cabbage, showing that the transcription level of BrULT2 gene was significantly down-regulated during the vegetative stage. We silenced the trxG family gene BrULT2 with optimized TYMV induced gene silencing, the TYMV-derived vector pTY was recombinated to infect Brassica rapa. After twenty days of inoculation, the infected plants showed abnormal phenotypes: leaf rolling, smaller and increased number leaves, thin stem, branch outgrowth. We detected that the expression level of BrULT2 gene of infected plants was downregulated meanwhile the transcriptional level of BrULT1 gene was up-regulated. Overall, our analysis introduce a novel mechanism that mediated BrULT family genes control the morphogenesis of Brassica rapa.

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