The Single-Stranded DNA-Binding Gene Whirly (Why1) with a Strong Pathogen-Induced Promoter from Vitis pseudoreticulata Enhances Resistance to Phytophthora capsici
文献类型： 外文期刊
作者： Lai, Chengchun 1 ; Que, Qiuxia 1 ; Pan, Ruo 1 ; Wang, Qi 1 ; Gao, Huiying 1 ; Guan, Xuefang 1 ; Che, Jianmei 2 ; Lai, Gongti 1 ;
作者机构： 1.Fujian Acad Agr Sci, Inst Agr Engn Technol, Fuzhou 350003, Peoples R China
2.Fujian Acad Agr Sci, Inst Agr Bioresources Res, Fuzhou 350003, Peoples R China
关键词： Whirly; promoter; Vitis pseudoreticulata; Phytophthora capsici; PR gene
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）
ISSN：
年卷期： 2022 年 23 卷 14 期
页码：
收录情况： SCI
摘要： Vitis vinifera plants are disease-susceptible while Vitis pseudoreticulata plants are disease-resistant; however, the molecular mechanism remains unclear. In this study, the single-stranded DNA- and RNA-binding protein gene Whirly (VvWhy1 and VpWhy1) were cloned from V. vinifera "Cabernet Sauvignon" and V. pseudoreticulata "HD1". VvWhy1 and VpWhy1 promoter sequences (pVv and pVp) were also isolated; however, the identity of the promoter sequences was far lower than that between the Why1 coding sequences (CDSs). Both Why1 gene sequences had seven exons and six introns, and they had a C-terminal Whirly conserved domain and N-terminal chloroplast transit peptide, which was then verified to be chloroplast localization. Transcriptional expression showed that VpWhy1 was strongly induced by Plasmopara viticola, while VvWhy1 showed a low expression level. Further, the GUS activity indicated pVp had high activity involved in response to Phytophthora capsici infection. In addition, Nicotiana benthamiana transiently expressing pVp::VvWhy1 and pVp::VpWhy1 enhanced the P. capsici resistance. Moreover, Why1, PR1 and PR10 were upregulated in pVp transgenic N. benthamiana leaves. This research presented a novel insight into disease resistance mechanism that pVp promoted the transcription of Why1, which subsequently regulated the expression of PR1 and PR10, further enhancing the resistance to P. capsici.
- 相关文献
作者其他论文 更多>>
-
Characterization and safety assessment of bamboo shoot shell cellulose nanofiber: Prepared by acidolysis combined with dynamic high-pressure microfluidization
作者：Luo, Xianliang;Wu, Yirui;Yang, Junyi;Chen, Peng;Zhuang, Weijing;Zheng, Yafeng;Wang, Qi;Liu, Wangxin
关键词：Bamboo shoot shell; Cellulose nanofiber; Characterization; Toxicology
-
Effects of a Mixture of Brevibacillus brevis with Other Bacillus sp. Strains against Gray Mold and on Enzyme Activities of Grape
作者：Che, Jianmei;Chen, Qianqian;Liu, Guohong;Liu, Bo;Lai, Chengchun;Lai, Gongti
关键词：antifungal activity; Brevibacillus brevis; gray mold disease; defense-related enzyme
-
The antioxidant capacity and nutrient composition characteristics of lotus (Nelumbo nucifera Gaertn.) seed juice and their relationship with color at different storage temperatures
作者：Zhang, Yanlin;Xu, Yixian;Zhang, Jingyuan;Dai, Xin;Lu, Xu;Wang, Qi;Miao, Song;Lu, Xu;Lu, Xu;Zhang, Yanlin;Xu, Yixian;Zhang, Jingyuan;Dai, Xin;Miao, Song;Lu, Xu;Lu, Xu
关键词：Lotus seeds; Antioxidants; Nutritional quality; Color; Phenolics; Storage; Kinetics
-
The modulation effect of lotus (Nelumbo nucifera Gaertn.) seeds oligosaccharides with different structures on intestinal flora and action mode of growth effects on Bifidobacterium in vivo and in vitro
作者：Ma, Xiaoqing;Zheng, Zhichang;Zuo, Jiaxin;Ju, Jianing;Zheng, Baodong;Lu, Xu;Wang, Qi;Zheng, Baodong;Lu, Xu;Ma, Xiaoqing;Zheng, Zhichang;Wang, Qi;Zuo, Jiaxin;Lu, Xu;Lu, Xu
关键词：Lotus seed; Oligosaccharides; Fecal gut microbiota; Prebiotics; Bifidobacterium; In vitro fermentation; Structures; Structure activity relationship
-
Curcumin-Loaded Bamboo Shoot Cellulose Nanofibers: Characterization and In Vitro Studies
作者：Chang, Yu;Luo, Xianliang;Huang, Yajuan;Wu, Yirui;Chen, Peng;Zheng, Yafeng;Wang, Qi;Huang, Juqing
关键词：cellulose nanofiber; bamboo shoot; curcumin; slow release
-
Characterization and in vitro assessment of probiotic potential of Lactiplantibacillus plantarum BXM2 from fermented honey passion fruit beverage
作者：Guan, Xuefang;Lin, Bin;Wang, Qi;Zhao, Dazhou;Huang, Junyang;Yang, Yijun;Zheng, Yafeng;Lin, Bin;Wang, Qi
关键词：antibacterial activity; fermentation; gastrointestinal tolerance; Lactiplantibacillus plantarum; probiotic
-
Structural characterization and in vitro analysis of the prebiotic activity of oligosaccharides from lotus (Nelumbo nucifera Gaertn.) seeds
作者：Lei, Yi;Zhang, Yanlin;Zheng, Baodong;Lu, Xu;Wang, Qi;Zheng, Baodong;Lu, Xu;Lei, Yi;Zhang, Yanlin;Zheng, Baodong;Miao, Song;Lu, Xu;Miao, Song
关键词：Lotus seeds; Oligosaccharides; Structures; Prebiotic
