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Molecular cloning, expression and functional analysis of three subunits of protein phosphatase 2A (PP2A) from black tiger shrimps (Penaeus monodon)

文献类型： 外文期刊

作者： Zhao, Chao 1 ; Wang, Yan 1 ; Fu, Mingjun 1 ; Yang, Keng 1 ; Qiu, Lihua 1 ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, 231 Xingangxi Rd, Guangzhou 510300, Guangdong, Peoples R China

2.Shanghai Ocean Univ, Coll Aqua Life Sci & Technol, Shanghai 201306, Peoples R China

3.Minist Agr, Key Lab South China Sea Fishery Resources Exploit, Guangzhou 510300, Guangdong, Peoples R China

4.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Trop Aquaculture Res & Dev Ct

关键词： Penaeus monodon; Protein phosphatase 2A; mRNA expression; 5-Hydroxytryptamine and dopamine; Phosphatase activity

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.231； 五年影响因子：2.215 ）

ISSN： 1096-4959

年卷期： 2017 年 204 卷

页码：

收录情况： SCI

摘要： Protein phosphatase 2A (PP2A) is a cellular serine-threonine (Ser/Thr) phosphatase that plays a crucial role in regulating most cellular functions. In the present study, the full-length cDNAs of three subunits of PmPP2A (PmPP2A-A, PP2A-B and PP2A-C) were cloned from Penaeus monodon, which are the first available for shrimps. Sequence analysis showed that PmPP2A-A, PmPP2A-B and PmPP2A-C encoded polypeptides of 591, 443, and 324 amino acids, respectively. The mRNAs of three subunits of PmPP2A were expressed constitutively in all tissues examined, and predominantly in the ovaries. In ovarian maturation stages, the three subunits of PmPP2A were continuously but differentially expressed. Dopamine and 5-hydroxytryptamine injection experiments were conducted to study the expression profile of three subunits of PmPP2A, and the results indicated that PmPP2A played a negative regulatory role in the process of ovarian maturation. In addition, the recombinant proteins of three subunits of PmPP2A were successfully obtained, and the phosphatase activity of PmPP2A was tested in vitro. The results of this study will advance our understanding about the molecular mechanisms of PmPP2A in Penaeus monodon. (C) 2016 Elsevier Inc. All rights reserved.

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