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Effects of cryopreservation at various temperatures on the survival of kelp grouper (Epinephelus moara) embryos from fertilization with cryopreserved sperm

文献类型： 外文期刊

作者： Tian, Yongsheng 1 ; Chen, Zhangfan 1 ; Tang, Jiang 1 ; Duan, Huimin 1 ; Zhai, Jieming 4 ; Li, Bo; Ma, Wenhui; Liu, 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266000, Peoples R China

3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

4.Ming Bo Aquat Co Ltd, Laizhou 261400, Pe

关键词： Epinephelus moara; Embryo cryopreservation; Frozen sperm

期刊名称：CRYOBIOLOGY （ 影响因子：2.487； 五年影响因子：2.641 ）

ISSN： 0011-2240

年卷期： 2017 年 75 卷

页码：

收录情况： SCI

摘要： Fish embryo cryopreservation is highly important for the long-term preservation of genomic and genetic information; however, few successful cases of fish embryo cryopreservation have been reported over the past 60 years. This is the first study to use Epinephelus moara embryos from fertilization with cryopreserved sperm as experimental material. Embryos that developed to the 16-22 somite stage and tail bud stage were treated with the vitrification solution PMG3T according to a five-step equilibration method and cryopreserved at various temperatures and storage duration. Only 19.9 +/- 9.2% of 16-22 somite stage embryos and 1.3 +/- 1.1% of tail-bud stage embryos survived when cooled at 4 degrees C for 60 min. In total, 8.0 +/- 3.0% of 16-22 somite stage embryos survived when cooled at-25.7 degrees C for 30 min, 22.4 +/- 4.7% of tail-bud stage embryos survived after 45 min of cooling at-25.7 degrees C, and none survived after 60 min. Only 2.0 +/- 2.7% of embryos survived when cryopreserved at-140 degrees C for 20 min. However, 9.7% of tail-bud stage embryos survived after cryopreservation in liquid nitrogen (-196 degrees C) for 2 h. Most surviving embryos developed normally. Embryonic volume decreased and spherical segments appeared when embryos were treated with higher concentrations of vitrification solution. Additionally, the volume recovered gradually after rinsing with sucrose' and seawater. This is the first estimate of the survival of E. moara embryos and larvae after cryopreservation. These findings provide a foundation for further explorations of fish embryo cryopreservation techniques. (C) 2017 Elsevier Inc. All rights reserved.

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