Characterization of emopamil binding protein (Cs-ebp) reveals its potential role in cholesterol biosynthesis and growth performance of Chinese tongue sole
文献类型： 外文期刊
作者： Chen, Zhangfan 1 ; Li, Xihong 1 ; Cheng, Peng 2 ; Mai, Jiaqi 2 ; Sun, Yuqi 2 ; Wang, Jiacheng 2 ; Shi, Rui 2 ; Zhang, Qi 2 ; Wang, Na 1 ; Chen, Songlin 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, State Key Lab Mariculture Biobreeding & Sustainabl, Qingdao 266071, Peoples R China
2.Chinese Acad Fishery Sci CAFS, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Marine Fisheries, Minist Agr, Qingdao 266071, Peoples R China
3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Shandong Key Lab Marine Fisheries Biotechnol & Gen, Qingdao 266071, Peoples R China
4.106 Nanjing RD, Qingdao 266071, Peoples R China
关键词： Chinese tongue sole; Emopamil-binding protein (EBP); Cholesterol biosynthesis; Sexual size dimorphism; Ovarian development
期刊名称：AQUACULTURE （ 影响因子：4.5； 五年影响因子：4.6 ）
ISSN： 0044-8486
年卷期： 2024 年 581 卷
页码：
收录情况： SCI
摘要： Sexual size dimorphism (SSD) in Chinese tongue sole affects its annual yields and consequently hinders its sustainable aquaculture development. Females show faster growth rate than male from 6 month post hatch (m). Comparative transcriptome analysis suggested cholesterol biosynthesis as an important signaling pathway involved in Chinese tongue sole SSD. Emopamil-binding protein (EBP), identified as one of the differential expressed genes in females, is the key catalyzing enzyme in the final steps of cholesterol biosynthesis. In this study, Chinese tongue sole ebp (Cs-ebp) gene was characterized and its potential role in cholesterol biosynthesis and growth performance was assessed. Cs-ebp showed significant abundance in female ovaries and livers. Meanwhile, it was highly expressed in 6 m female ovaries compared to other developmental stages. Promoter analysis revealed that transcriptional factors JUNB (a subunit of the activating protein-1 transcription factor complex) and POU1F1 alpha (Pou-homeodomain transcription factor) could promote Cs-ebp transcription. Cs-ebp gene knockdown triggered the down-regulation of its downstream genes in cholesterol biosynthesis pathway (sc5d, dhcr7, and dhcr24), and genes related to estrogen biosynthesis (cyp19a1a) and body growth (akt1 and bmp2b). All the results revealed that there might be crosstalk among Cs-ebp, PI3K/Akt, TGF-beta/Bmp, and estrogen biosynthesis, eventually affected the growth performance of female Chinese tongue sole. Further investigation would provide us detailed information about the molecular mechanism beneath teleost SSD.
- 相关文献
作者其他论文 更多>>
-
Differentially expressed proteins and microbial communities of the skin regulate disease resistance to Chinese tongue sole (Cynoglossus semilaevis)
作者：Wang, Lei;Tian, Min;Chen, Songlin;Wang, Lei;Tian, Min;Chen, Songlin;Wang, Lei;Tian, Min;Chen, Songlin;Tian, Min
关键词：Cynoglossus semilaevis; skin; proteome; microbial community; protein microbial interaction
-
Establishment, characterization and application of Largemouth bronze gudgeon ( Coreius guichenoti ) fin and skin cell lines in disease resistance
作者：Zhang, Qi;Shi, Yuhong;Liao, Xiaolin;Tian, Hua;Zhang, Qi;Gong, Zhihong;Shi, Rui;Shi, Yuhong;Guo, Chenfei;Wang, Na;Gong, Zhihong
关键词：Largemouth bronze gudgeon(Coreius guichenoti); Cell lines; Skin; Fin; Spring viremia of carp virus (SVCV)
-
Integrated transcriptomic and metabolomic analysis provides insights into the responses to Vibrio infection in Plectropomus leopardus
作者：Wang, Jie;Zhang, Junwei;Chen, Songlin;Zhou, Qian;Wang, Jie;Zhu, Chunhua;Wang, Shengpeng;Wang, Shengpeng
关键词：Plectropomus leopardus; Vibrio infection; Transcriptomics; Metabolomics; Bile acids; Eicosanoids
-
Establishment and characterization of cell line from fin of Chinense bahaba (Bahaba taipingensis) and transcriptome analysis of its response to megalocytivirus SKIV-SD strain infection
作者：Gong, Zhihong;Zhang, Qi;Wang, Na;Wang, Lei;Chen, Zhangfan;Chen, Songlin;Gong, Zhihong;Hu, Guobin;Zhang, Qi;Yan, Lin;Yan, Kuoqiu
关键词：Chinese bahaba; Fin; Cell line; BTF; Megalocytivirus
-
The potential role of hippo pathway effector yap1/yap1b in female-biased sexual size dimorphism of Chinese tongue sole (Cynoglossus semilaevis)
作者：Mai, Jiaqi;Mai, Jiaqi;Sun, Yuqi;Li, Xihong;Xu, Wenteng;Chen, Zhangfan;Zhang, Qi;Wang, Jiacheng;Wang, Na;Li, Xihong;Xu, Wenteng;Chen, Zhangfan;Wang, Na;Zhu, Ying
关键词：Cynoglossus semilaevis; Sexual size dimorphism; Hippo signaling pathway; yap1; yap1b
-
Effects of Inducible Nitric Oxide Synthase (iNOS) Gene Knockout on the Diversity, Composition, and Function of Gut Microbiota in Adult Zebrafish
作者：Huang, Yajuan;Chen, Yadong;Xie, Haisheng;Feng, Yidong;Bao, Baolong;Chen, Yadong;Chen, Songlin
关键词：inducible nitric oxide synthase; zebrafish; gut microbiota; 16S rRNA; immune
-
Integrative omics reveals clues as to why Cynoglossus semilaevis pseudomales produce no W sperm
作者：Zhang, Yaqun;Wang, Liping;Xu, Feng;Ye, Zhan;Li, Hengde;Zhang, Yaqun;Wang, Liping;Xu, Feng;Ye, Zhan;Li, Hengde;Wang, Liping;Xu, Feng;Ye, Zhan;Chen, Songlin;Chen, Songlin;Chen, Songlin;Li, Hengde
关键词：Spermatogenesis; Transcriptome; Proteome; Ubiquitination; Pseudomale; Cynoglossus semilaevis
