Physicochemical and Reactive Oxygen Species Scavenging Properties of Collagen and Collagen Hydrolysates from Farmed Globefish (Fugu rubripes) Bone
文献类型： 外文期刊
作者： Wang, Shanshan 1 ; Sun, Xiaoqi 1 ; Zhou, Deqing 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Dept Food Engn & Nutr, 106 Nanjing Rd, Qingdao 266071, Shandong, Peoples R China
关键词： Globefish bone; collagen; fibril morphology; SEM; hydrolysis; ROS
期刊名称：JOURNAL OF AQUATIC FOOD PRODUCT TECHNOLOGY （ 影响因子：1.767； 五年影响因子：1.628 ）
ISSN： 1049-8850
年卷期： 2017 年 26 卷 5 期
页码：
收录情况： SCI
摘要： Globefish (Fugu rubripes) bone collagen (GBC) was isolated and characterized. Electrophoresis and Fourier transform infrared (FTIR) spectra indicated that GBC was high-purity type I collagen with an intact triple-helical structure. Amino acid analysis revealed that GBC contained higher glycine content (364 residues/1,000 residues) than that of other fishery bones, and the imino acid content was 189 residues/1,000 residues. The denaturation temperature (T-d) of GBC was 27.1 degrees C. GBC displayed a rapid fibril-forming rate and well-defined fibril morphology in vitro. Globefish bone collagen hydrolysates (GBCH) were optimized by neutral protease through response surface methodology (RSM) with the highest hydroxyl radical (center dot OH) scavenging capacity. The optimal hydrolysis condition was a reaction temperature of 48.4 degrees C, time of 120 min, and enzyme/substrate ratio of 2520 U/g. Three fractions were collected by ultrafiltration, and the fraction GBCH-III (Mw < 1 kDa) possessed the strongest center dot OH scavenging ability. Reactive oxygen species (ROS) scavenging capacities were evaluated with two model systems: center dot OH and nitric oxide (NO center dot). The ROS scavenging capacities increased in a dose-dependent manner. GBCH-III proved to be a more effective center dot OH scavenger than reduced glutathione (GSH). These findings demonstrated that GBC and GBCH have potential as an alternative source of collagen-derived materials for use in pharmaceutical, nutraceutical, and cosmetic industries.
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