您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Gold Nanoparticle-Decorated Single-Walled Carbon Nanotubes as a Catalytic Amplification Platform for the Electrochemical Detection of Alkaline Phosphatase Activity

文献类型： 外文期刊

作者： Zhao, Lingzhi 1 ; Zhao, Liu 3 ; Miao, Yanqing 1 ; Zhang, Chenxiao 2 ;

作者机构： 1.Xian Med Coll, Coll Pharm, Xian 710021, Shaanxi, Peoples R China

2.Shaanxi Normal Univ, Key Lab Analyt Chem Life Sci Shaanxi Prov, Sch Chem & Chem Engn, Xian 710062, Shaanxi, Peoples R China

3.Beijing Res Ctr Agr Stand & Testing, Beijing 100097, Peoples R China

关键词： Alkaline phosphatase activity; Gold particles; Single-walled carbon nanotubes (SWNTs); whole cell lysate

期刊名称：INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE （ 影响因子：1.765； 五年影响因子：1.685 ）

ISSN： 1452-3981

年卷期： 2018 年 13 卷 2 期

页码：

收录情况： SCI

摘要： The detection of alkaline phosphatase (ALP) activity is of great significance in many biomedical applications and for understanding the functional mechanism of ALP-related biological events. A sensitive electrochemical ALP biosensor using ascorbic acid 2-phosphate (AA-P) as the substrate was developed based on a gold nanoparticle-decorated single-walled carbon nanotube (GNP/SWNT)-modified glassy carbon electrode (GCE). The activity of ALP was determined indirectly according to the concentration of ascorbate (AA), which was generated during the hydrolysis reaction of AA-P in the presence of ALP as a catalyst. The biosensor exhibited a low applied potential, high sensitivity and selectivity, and the current response increased with ALP concentration from 3 to 50 U L-1 with a detection limit of 0.2 U L-1. The applicability of the developed method was demonstrated for successfully detecting ALP in whole HeLa cell lysate, thus providing a promising tool for other sensing systems that involve ALP.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台