A Naked-Eye Visible and Fluorescence "Turn-On" Probe for Acetylcholinesterase Assay and the Discrimination of Biothiols
文献类型： 外文期刊
作者： Zhao, Lingzhi 1 ; Zhao, Liu 3 ; Zhang, Chenxiao 2 ; Li, Yanqi 2 ;
作者机构： 1.Xian Med Coll, Xian 710021, Shaanxi, Peoples R China
2.Shaanxi Normal Univ, Sch Chem & Chem Engn, Xian 710062, Shaanxi, Peoples R China
3.Beijing Res Ctr Agr Stand & Testing, Beijing 100097, Peoples R China
关键词： 4-chloro-7-nitrobenzofurazan; colorimetry; fluorescence; reduced biothiols; acetylcholin-esterase; paraoxon
期刊名称：JOURNAL OF APPLIED SPECTROSCOPY （ 影响因子：0.741； 五年影响因子：0.718 ）
ISSN： 0021-9037
年卷期： 2018 年 85 卷 3 期
页码：
收录情况： SCI
摘要： We demonstrate the utility of 4-chloro-7-nitrobenzofurazan (NBD-Cl), a simple, low-cost probe, for colorimetrically and fluorescently detecting and differentiating similar thiol species, including cysteine, homocysteine, and glutathione, as well as for assaying acetylcholinesterase (AChE) activity. This assay is a potential tool for investigating thiol functions in disease and diagnosis, and for screening AChE inhibitors. We envision that this versatile probe, which is commercially available and inexpensive, will be useful for further elucidating the roles of thiols in biology and also shows applicability for analysis of AChE inhibitors in physiological samples.
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