Colorimetric Sensing of Cysteine in serum by the competitive adsorption toward AuNPs between Cysteine and ATP
文献类型： 外文期刊
作者： Zhao, Lingzhi 1 ; Zhao, Liu 2 ; Miao, Yanqing 1 ; Liu, Chunye 1 ;
作者机构： 1.Xian Med Coll, Dept Pharm, Xian 710021, Peoples R China
2.Beijing Res Ctr Agr Stand & Testing, Beijing 100097, Peoples R China
3.Shaanxi Normal Univ, Sch Chem & Chem Engn, Key Lab Analyt Chem Life Sci Shaanxi Prov, Xian 710062, Peoples R China
关键词： colorimetric;cysteine;reduced glutathione;homocysteine;Gold nanoparticles (AuNPs)
期刊名称：PROCEEDINGS OF THE 3RD INTERNATIONAL CONFERENCE ON MATERIAL, MECHANICAL AND MANUFACTURING ENGINEERING
ISSN： 2352-5401
年卷期： 2015 年 27 卷
页码：
收录情况： SCI
摘要： Based on the dependence between the AuNPs size and the naked-eye visible color change. This study was designed to develop a highly selective and sensitive towards colorimetric sensing of cysteine in fetal bovine serum by the competitive binding of ATP and thiol compounds (cysteine and homocysteine) towards gold nanoparticles (AuNPs). The as-established method has high sensitivity and selectivity, the linearity range was 3 mu M-30 mu M with the quantification limit of 1 mu M. The method demonstrated here can be applied to detect cysteine in fetal bovine serum without interference from other amino acids and bovine serum albumin (BSA), which is envisaged to be applicable to understanding the chemical essence involved in physiological and pathological events associated with cysteine.
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