文献类型： 外文期刊
作者： Chen, Yu 1 ; Cai, Qinan 2 ; Hao, Shuiyuan 3 ;
作者机构： 1.Nanjing Agr Univ, Dept Hort, Nanjing 210095, Jiangsu, Peoples R China
2.Jilin Acad Agr Sci, Agrobiotechnol Res Inst, Changchun 130124, Jilin, Peoples R China
3.Hetao Coll, Dept Agr Sci, Bayannur City 015000, Peoples R China
关键词： Brevis plant1; Inositol polyphosphate 5-phosphatase; Stem elongation
期刊名称：JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY （ 2020影响因子：1.175； 五年影响因子：1.238 ）
ISSN： 0971-7811
年卷期： 2018 年 27 卷 2 期
收录情况： SCI
摘要： The genes associated with the dwarf phenotype have been utilized in crop breeding to prevent lodging and stem breakage. Brevis plant1 (Bv1), encoding a putative inositol polyphosphate 5-phosphatase (5PTase), has been associated with stem elongation in maize (Zea mays L.); however, the enzymatic activity of BV1 has not been experimentally characterized. In this study, the phosphatase activity of BV1 was verified with biochemical assays. BV1 demonstrated type II 5PTase activity capable of hydrolyzing both inositol polyphosphates and phosphoinositides. Similar to other type II 5PTases that share similar sequences and common domain architecture with BV1, the enzymatic activity of BV1 is sensitive to changes in Mg2+ and pH. 367G-R and 565S-L are two mutations in Bv1 that have been associated with the dwarfing phenotype in maize. To characterize the dwarfing mechanism, mutant BV1 proteins were expressed in vitro and assayed for phosphatase activity. The results showed that both mutations significantly reduced the enzymatic activity of BV1, but neither altered its substrate specificity and dependence on Mg2+ and pH. This biochemical verification of BV1 as a type II 5PTase is important as it sheds light on the molecular basis of deficient BV1-mediated dwarfing phenotype.
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