您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Effects of dietary cystine and tyrosine on melanogenesis pathways involved in skin color differentiation of Malaysian red tilapia

文献类型： 外文期刊

作者： Wang, Lan-mei 1 ; Zhu, Wen-bin 1 ; Yang, Jie 2 ; Miao, Ling-hong 1 ; Dong, Juan-juan 2 ; Song, Fei-biao 2 ; Dong, Zai- 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Wuxi 214081, Peoples R China

2.Nanjing Agr Univ, Wuxi Fisheries Coll, Wuxi 214128, Peoples R China

关键词： Red tilapia; Dietary cystine and tyrosine; Melanin; Tyrosinase; slc7a11 gene

期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）

ISSN： 0044-8486

年卷期： 2018 年 490 卷

页码：

收录情况： SCI

摘要： A 68-day experiment was conducted to investigate the effects of dietary cystine (Cys) and tyrosine (Tyr) on skin melanogenesis pathways in Malaysian red tilapia (initial weight: 15.04 +/- 0.03 g). Six practical diets containing extra 0.1%, 0.5%, 1% cystine (Cys) and 0.5%, 1%, 2% tyrosine (Tyr) based on the control diet were prepared, accordingly named as Control, Cys-0.1%, Cys-0.5%, Cys-1%, Tyr-0.5%, Tyr-1% and Tyr-2% respectively. Each diet was fed to triplicate groups of fish during the trial. At the end of the experiment, the final weight for each group was not significantly different (P > 0.05). However, the dietary Cys and Tyr had significant effects on the melanin level and tyrosinase activity in the skin of fish. The melanin content in the skin of fish was lowest in Cys-0.5% group, with a higher level observed at Cys-0.1% and Cys-1% group. The final melanin of fish skin in Cys-1% was higher than that in Cys-0.5% and Cys-0.1% (P < 0.05). At the end of the experiment, the melanin in ventral skin of fish in Tyr-2% was higher than that in Tyr-0.5% and Tyr-1% (P < 0.05), and the melanin in dorsal skin of fish in Tyr-0.5% was lower than that in Tyr-1% and Tyr-2% (P < 0.05). The final tyrosinase activity of fish skin in Cys-1% was highest, and the final tyrosinase activity in Tyr-1% and Tyr-2% were significantly higher than that in Tyr-0.5% (P < 0.05). In serum, the tyrosinase activity was highest in Cys-1%, and the tyrosinase activity in Tyr-2% was significantly higher than that in Tyr-1% and Tyr-0.5% (P < 0.05). Moreover, the ventral skin melanin zone in Cys-1% treatment is largest by slice microstructure observation. The expression of tyrosinase mRNA in Tyr-2% group was highest in ventral skin of fish. At the end of the experiment, tyrosinase mRNA in the dorsal skin of fish was lowest in Cys-0.5%, with a higher level observed at Cys-0.1% and Cys-1% group. The final expression of tyrosinase mRNA in Tyr-0.5% was lower than that in Tyr-1% and Tyr-2% in dorsal skin of fish (P < 0.05). The final expressions of slc7a11 mRNA in Tyr-1% was higher than that in Tyr-0.5% in dorsal skin of fish (P < 0.05). Present results demonstrated that dietary Cys and Tyr could affect the melanin synthesis pathway in the red tilapia.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台