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Proteomic and Phosphoproteomic Analyses Reveal Gibel Carp Responses to Cyprinid Herpesvirus 2 Infection

文献类型： 外文期刊

作者： Xu, Chen 1 ; Li, Yiqun 1 ; Xiao, Zidong 1 ; Yang, Jie 1 ; Xue, Mingyang 1 ; Jiang, Nan 1 ; Meng, Yan 1 ; Liu, Wenzhi 1 ; Fan, Yuding 1 ; Zhou, Yong 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

2.Huazhong Agr Univ, Coll Fisheries, Wuhan 430072, Peoples R China

3.Jiangxi Agr Univ, Coll Biol Sci & Engn, Nanchang 330045, Peoples R China

关键词： CyHV-2; HVHND; quantitative proteomics; phosphoproteomic; virus-host interaction; gibel carp

期刊名称：JOURNAL OF PROTEOME RESEARCH （ 影响因子：5.37； 五年影响因子：4.995 ）

ISSN： 1535-3893

年卷期： 2022 年 21 卷 8 期

页码：

收录情况： SCI

摘要： Cyprinid herpesvirus 2 (CyHV-2) is a typical linear double-stranded DNA virus, which can induce severe herpesviral hematopoietic necrosis disease (HVHND) in gibel carp. However, the CyHV-2 infection mechanisms still remain unresolved till now. Here, we combined the isobaric tag for relative absolute quantitation (iTRAQ)-labeled quantitative proteomic and phosphoproteomic analysis enriched by Ti4+-immobilized titanium ion affinity chromatography (IMAC) to uncover the host responses to CyHV-2 infection in the kidneys of symptomatic and diseased gibel carp. We totally identified 192 differential expression proteins and 951 high-confident phosphopeptides involved in 657 proteins. After being infected with CyHV-2, the proteins involved in energy generation and ion balance were significantly downregulated in the host, and the phosphorylated proteins induced by viral infection mainly participated in the regulation for RNA processing, translation, cytoskeleton organization, immunoreaction, etc. Furthermore, 11 phosphorylated CyHV-2 viral proteins were found in the diseased group by the host proteome. The virus-host protein-protein interactions were investigated, in which the potential host kinases casein kinase II (CK-II) and cyclin-dependent kinase (CDK) that interacted with viral ORF88 or ORF89 were identified and can serve as candidate targets for disease treatment in the future. Overall, our study provides a comprehensive understanding of CyHV-2-induced perturbations at the protein and phosphorylation levels in gibel carp, forming a base for the treatment of HVHND.

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