Generation, safety and immunogenicity of an Actinobacillus pleuropneumoniae quintuple deletion mutant SLWO7 (Delta apxIC Delta apxIIC Delta orf1 Delta cpxAR Delta arcA)
文献类型： 外文期刊
作者： Yuan, Fangyan 2 ; Liu, Jinlin 4 ; You, Wujin 3 ; Bei, Weicheng 3 ; Wang, Chunlai 1 ; Zhao, Jin 5 ; Tian, Yiongxiang 2 ; L 1 ;
作者机构： 1.Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Heilongjiang, Peoples R China
2.Hubei Acad Agr Sci, Inst Anim Husb & Vet Sci, Key Lab Prevent & Control Agents Anim Bacteriosis, Minist Agr,Hubei Key Lab Anim Embryo & Mol Breedi, Wuhan 430064, Hubei, Peoples R China
3.Huazhong Agr Univ, Coll Vet Med, Cooperat Innovat Ctr Sustainable Pig Prod, State Key Lab Agr Microbiol, Wuhan 430070, Hubei, Peoples R China
4.Cent China Normal Univ, Coll Life Sci, Wuhan 430079, Hubei, Peoples R China
5.Cent China Normal Univ, Coll Life Sci, Wuhan 430079
关键词： Actinobacillus pleuropneumoniae; Quintuple deletion mutant; Safety; Protective efficacy
期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）
ISSN： 0264-410X
年卷期： 2018 年 36 卷 14 期
页码：
收录情况： SCI
摘要： We inactivated a virulence determinant, ArcA, in an Actinobacillus pleuropneumoniae quadruple deletion mutant SLW06 (Delta apxIC Delta apxIIC Delta orf1 Delta cpxAR, serovar 1), and a quintuple deletion mutant SLW07 was generated. SLW07 showed decreased adherence to and invasion of host cells, compared to its parent strain SLW06. SLW07 was more sensitive in RAW264.7 macrophage-mediated phagocytosis and clearance. SLW07 was less virulent in mice. An immunization assay indicated that both SLW07 and SLW06 preferentially stimulated T helper cell type 2 response in mice. Live vaccines induced the production of interleukin-6 and tumor necrosis factor-alpha by splenic lymphocytes. Furthermore, the protective immunity of SLW07 was not affected after ArcA mutation. Immunization with SLW07 could provide a complete protection following virulent A. pleuropneumoniae challenge in mice. Our results suggest that SLW07 is a promising live vaccine candidate, which is further attenuated from and shares similar protective efficacy with its quadruple deletion parent SLW06. (C) 2018 Elsevier Ltd. All rights reserved.
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