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Development of Certified Matrix-Based Reference Material as a Calibrator for Genetically Modified Rice G6H1 Analysis

文献类型： 外文期刊

作者： Yang, Yu 1 ; Li, Liang 2 ; Yang, Hui 1 ; Li, Xiaying 3 ; Zhang, Xiujie 3 ; Xu, Junfeng 4 ; Zhang, Dabing 1 ; Jin, Wujun 2 ;

作者机构： 1.Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Natl Ctr Mol Characterizat Genet Modified Organis, Shanghai 200240, Peoples R China

2.Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

3.Minist Agr Peoples Republ China, Dev Ctr Sci & Technol, Beijing 100025, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Qual & Standard Agroprod, State Key Lab Breeding Base Zhejiang Sustainable, Hangzhou 310021, Zhejiang, Peoples R China

关键词： genetically modified organism; certified reference materials; G6H1 rice; characterization value

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2018 年 66 卷 14 期

页码：

收录情况： SCI

摘要： The accurate monitoring and quantification of genetically modified organisms (GMOs) are key points for the implementation of labeling regulations, and a certified reference material (CRM) acts as the scaleplate for quantifying the GM contents of foods/feeds and evaluating a GMO analytical method or equipment. Herein we developed a series of CRMs for transgenic rice event G6H1, which possesses insect-resistant and herbicide-tolerant traits. Three G6H1 CRMs were produced by mixing seed powders obtained from homozygous G6H1 and its recipient cultivar Xiushui 110 at mass ratios of 49.825%, 9.967%, and 4.986%. The between-bottle homogeneity and within-bottle homogeneity were thoroughly evaluated with consistent results. The potential DNA degradation in transportation and shelf life were evaluated with an expiration period of at least 12 months. The property values of three CRMs (G6H1(a), G6H1(b), G6H1(c)) were given as (49.825 +/- 0.448) g/kg, (9.967 +/- 1.757) g/kg, and (4.986 +/- 1.274 g/kg based on mass fraction ratio, respectively. Furthermore, the three CRMs were characterized with values of (5.01 +/- 0.08)%, (1.06 +/- 0.22)%, and (0.53 +/- 0.11)% based on the copy number ratio using the droplet digital PCR method. All results confirmed that the produced G6H1 matrix-based CRMs are of high quality with precise characterization values and can be used as calibrators in GM rice G6H1 inspection and monitoring and in evaluating new analytical methods or devices targeting the G6H1 event.

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