文献类型： 外文期刊
作者： Zhang, X. 1 ; Zhang, D. 2 ; Wang, L. 2 ; Liu, D. 1 ;
作者机构： 1.Northeast Agr Univ, Coll Anim Sci & Technol, Harbin, Heilongjiang, Peoples R China
2.Heilongjiang Acad Agr Sci, Harbin, Heilongjiang, Peoples R China
关键词： C2C12 cell; Min pig; myogenin gene; promoter
期刊名称：ACTA AGRICULTURAE SCANDINAVICA SECTION A-ANIMAL SCIENCE （ 影响因子：0.684； ）
ISSN： 0906-4702
年卷期： 2017 年 67 卷 1-2 期
页码：
收录情况： SCI
摘要： Myogenin (MyoG) plays an important role in muscle differentiation, which can initiate the formation of the fusion of muscle cells and myofibroblasts. To investigate the transcriptional regulatory mechanism of MyoG gene in Min pig, the promoter fragment spanning from -2085bp to +118bp was amplified with PCR, and the potential transcriptional elements were predicted via bioinformatics analysis. Subsequently, nine promoter segments with different base pair sizes were obtained and ligated into PGL3-Basic vectors, respectively. Lastly, the functional activity of each of the aforementioned promoters was tested separately in transiently transfected C2C12 cells via a dual-luciferase assay system. The results indicated that the sequence -199bp to -31bp may have the basic activity of the promoter. Further analysis demonstrated that the sequence -137bp to -101bp was important for the basic transcriptional activity. Bioinformatics analysis showed that CdxA and MZF1 transcription factors may play an important role in the regulation of MyoG expression.
- 相关文献
作者其他论文 更多>>
-
ANTIMICROBIAL ACTIVITY AND COMPONENT ANALYSIS OF CLEOME SPINOSA AGAINST FUSARIUM OXYSPORUM
作者：Zhang, X. Z.;Wang, C. L.;Liu, D.;Sa, R. N.;Gao, J. Y.;Liu, X. F.;Liu, D. W.;Yang, S.;Ma, T.;Li, X. L.;Chen, R. X.;Du, H. R.;Zhang, Y. J.;Zhang, X. Z.;Sa, R. N.
关键词：Antimicrobial activity; Cleome spinosa; Fusarium oxysporum; GC-MS; Volatile constituent identification Published first online January 24; 2021; Published final August 07; 2021
-
Occurrence of Leaf Spot of Begonia semperflorens Caused by Alternaria tenuissima in China
作者：Zhang, X.;Wang, R. T.;Li, Y. G.;Jiang, B. W.;Sun, L.
关键词：Begonia sempetflorens; Alternaria tenuissima; leaf spot
-
First Report of Leaf Spot of Cornus alba Caused by Botryosphaeria dothidea in China
作者：Cai, Y. N.;Zhang, X.;Li, Y. G.;Wang, K. Q.
关键词：Cornus alba; Botryosphaeria dothidea; leaf spot; ornamental plant
-
First Report of Rhizoctonia solani AG-1-IB Infecting Potato with Stem Canker in Heilongjiang Province, China
作者：Yang, S.;Min, F.;Zhang, J.;Hu, L.;Kong, Y.;Wang, L.;Wang, X.
关键词：Rhizoctonia solani AG-1-IB; potato; first report
-
Identification of differentially expressed genes in adipose tissue of min pig and large white pig using RNA-seq
作者：Wang, W. T.;Liu, D.;Wang, W. T.;Zhang, D. J.;Liu, Z. G.;Peng, F. G.;Wang, L.;Fu, B.;Wu, S. H.;Li, Z. Q.;Guo, Z. H.;Liu, D.
关键词：RNA-seq; min pig; large white pig; fat; differentially expressed genes (DEGs)
-
Design of Multisine Phase References/Standards for Modulated Measurements
作者：Zhang, Y.;Huang, J.;Guo, X.;He, Z.;Wang, L.;Zhao, W.;Zhang, Z.;Lu, Z.
关键词：phase reference; phase standard; multisine; Nonlinear Vector Network Analyzer (NVNA); modulated signal
-
MICROBIAL ACTIVITY AND COMMUNITY DIVERSITY IN TOBACCO RHIZOSPHERIC SOIL AFFECTED BY DIFFERENT PRE-CROPS
作者：Li, X.;Zhang, X.;Yue, B.;Sun, G.;Li, X.;Zhang, H.;He, G.;Xu, N.;Sun, M.;Zhao, Y.
关键词：soybean;maize;monocropping;rotation;bacterial community structure;bacterial community function
