文献类型： 外文期刊

作者： Zhang, X. Z. ¹ ; Wang, C. L. ¹ ; Liu, D. ¹ ; Sa, R. N. ¹ ; Gao, J. Y. ¹ ; Liu, X. F. ¹ ; Liu, D. W. ¹ ; Yang, S. ¹ ; Ma, T. ¹ ; Li, X. L. ¹ ; Chen, R. X. ¹ ; Du, H. R. ¹ ; Zhang, Y. J. ¹ ;

作者机构： 1.Northeast Agr Univ, Coll Agron, Harbin, Peoples R China

2.Heilongjiang Acad Agr Sci, Mudanjiang Branch, Mudanjiang, Peoples R China

3.Heilongjiang Acad Agr Sci, Harbin, Peoples R China

关键词： Antimicrobial activity; Cleome spinosa; Fusarium oxysporum; GC-MS; Volatile constituent identification Published first online January 24; 2021; Published final August 07; 2021

期刊名称：JOURNAL OF ANIMAL AND PLANT SCIENCES-JAPS （ 影响因子：0.57； 五年影响因子：0.756 ）

ISSN： 1018-7081

年卷期： 2021 年 31 卷 5 期

页码：

收录情况： SCI

摘要： Cucumber Fusarium wilt is an important soil-borne disease that restricts cucumber production in all areas of the world. To explore the preventive effects of Cleome spinosa on cucumber Fusarium wilt, five different doses of Cleome spinosa powder including 0, 0.1, 0.2, 0.3, and 0.4 g/dish were applied to cucumber plant infected with Fusarium oxysporum f.sp. cucumarinum at Northeast Agricultural University. The data thus collected on various parameters were subjected to oneway analysis of variance (ANOVA) under Completely Randomized Design (CRD). The difference in treatment means were separated using Duncan's Multiple Range (DMR) Test. A 100% inhibition rate on F. oxysporum mycelium was achieved when the dose of C. spinosa powder rose to 0.3 or 0.4 g/dish. As the concentration of the Cleome spinosa extract increased, the inhibitory effects on diameters and dry weight of mycelium also increased. Median inhibitory concentration of C. spinosa on mycelium was was 45.12 mg mL-1. Gas chromatography-mass spectrometry identified twenty-one sorts of volatile constituents of Cleome spinosa, including heterocyclic compounds, alcohol, chromene, ester, acid and long chain alkanes. Twelve components of F. oxysporum were extracted by fumigation. Among those components, paeonol, linalool and theaspirane had antimicrobial activities against F. oxysporum, with inhibition rates of 73.9%, 75.9% and 80.4%, respectively. However, tetradecyl-oxirane, tetracosane, heptacosane, 3-ethyl-2-hydroxy-2cyclopenten-1-one, octacosane, 1,1,4A-trimethyl-3,4,4A,5,6,7-hexahydro-1H-naphthalen-2-one,6,10,14-trimethyl-2pentadecanone, methyl alpha-linolenate and pentatriacont-17-ene had no inhibitory effect against F. oxysporum. Those results suggested that the components of Cleome spinosa powder could effectively restrain cucumber Fusarium wilt.