文献类型： 外文期刊
作者： He, X. -M. 1 ; Du, X. 1 ; Zhuo, J. -S. 1 ; Jing, X. -Y. 1 ; Yang, X. -Q. 1 ; Liu, D. 1 ;
作者机构： 1.Northeast Agr Univ, Coll Anim Sci Technol, Harbin, Heilongjiang, Peoples R China
2.Heilongjiang Acad Agr Sci, Harbin, Heilongjiang, Peoples R China
关键词： ISG58; promoter; ISRE; NF-B; transient transfection
期刊名称：ACTA AGRICULTURAE SCANDINAVICA SECTION A-ANIMAL SCIENCE （ 影响因子：0.684； ）
ISSN： 0906-4702
年卷期： 2017 年 67 卷 1-2 期
页码：
收录情况： SCI
摘要： Interferon (IFN)-stimulated gene (ISG) 56 family (composed of ISG54, ISG56, ISG58, and ISG60) plays important roles in defense against viral infection in mammalian cells. Numerous studies have been conducted on ISG54, ISG56, and ISG60; however, little is known on ISG58. In the present study, the upstream sequence of porcine ISG58 gene was first characterized as functional promoter by luciferase reporter assay, and then two directly adjacent IFN-stimulated response elements (ISREs), one at -206 to -194 (ISRE-I) and a second one, directly upstream of this element at -219 to -207bp (ISRE-II), were identified using the bioinformatics method. The subsequent site-directed deletion and transient transfection experiments showed the candidate ISREs are functional. ISRE-I works better than ISRE-II and synergistic cooperation exists between two ISREs. Additionally, the effect of porcine ISG58 on activation of NF-B was analyzed using the dual-luciferase reporter assay. The results will contribute to revealing the role of ISG58 in immune response.
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