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The CD163 long-range scavenger receptor cysteine-rich repeat: expression, purification and X-ray crystallographic characterization

文献类型： 外文期刊

作者： Li, Rui 1 ; Ma, Hongfang 1 ; Jiang, Longguang; Qiao, Songlin 1 ; Zhi, Yubao 1 ; Huang, Mingdong; Deng, Ruiguang; 1 ;

作者机构： 1.Henan Acad Agr Sci, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Minist Agr, 116 Huayuan Rd, Zhengzhou 450002, Henan, Peoples R China

2.Henan Agr Univ, Coll Anim Sci & Vet Med, 63 Nongye Rd, Zhengzhou 450002, Henan, Peoples R China

3.Henan Agr Univ, Coll Anim Sci & Vet Med, 63 Nongye Rd, Zh

关键词： scavenger receptor; long-range scavenger receptor cysteine-rich repeat; SRCR repeat; CD163; X-ray crystallography

期刊名称：ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS （ 影响因子：1.056； 五年影响因子：1.089 ）

ISSN： 2053-230X

年卷期： 2018 年 74 卷

页码：

收录情况： SCI

摘要： Scavenger receptors (SRs) play critical roles in various physiological and pathological pathways. One of them, CD163, is a multifunctional endocytic receptor and is characterized by a long-range scavenger receptor cysteine-rich (SRCR) repeat. However, the structural and functional details of this long-range SRCR repeat have not yet been elucidated. In this study, the CD163 long-range SRCR repeat was expressed in Drosophila Schneider 2 cells. The recombinant protein was homogeneous after purification by metal-affinity, cation-exchange and size-exclusion chromatography. Single crystals were obtained using 20% PEG 4000, 0.15 M potassium sodium tartrate tetrahydrate pH 8.5 and diffracted to 3.30 angstrom resolution. As the first view of a long-range SRCR repeat, this work lays the structural basis for a deep understanding of SRs and their multiple functions.

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