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Discovery and Characterization of a Novel Chitosanase from Paenibacillus dendritiformis by Phylogeny-Based Enzymatic Product Specificity Prediction

文献类型： 外文期刊

作者： Sun, Huihui 1 ; Mao, Xiangzhao 2 ; Guo, Na 2 ; Zhao, Ling 1 ; Cao, Rong 1 ; Liu, Qi 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Dept Food Engn & Nutr, Qingdao 266071, Peoples R China

2.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China

关键词： chitosanase; (GlcN)(2); chitosan; Paenibacillus dendritiformis; product specificity prediction

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2018 年 66 卷 18 期

页码：

收录情况： SCI

摘要： In the process of genome mining for novel chitosanases by phylogeny-based enzymatic product specificity prediction, a gene named Csn-PD from Paenibacillus dendritiformis was discovered. The enzyme was classified as a member of the GH46 family of glycoside hydrolase based on sequence alignment, and it was functionally expressed in Escherichia coli BL21 (DE3). The recombinant chitosanase was purified, and its molecular weight was estimated to be 31 kDa by SDS-PAGE. Csn-PD displayed maximal activity toward colloidal chitosan at pH 7.0 and 45 degrees C, respectively. A combination of thin-layer chromatography and electrospray ionization-mass spectrometry results showed that Csn-PD exhibited an endotype cleavage pattern and hydrolyzed chitosan to yield (GlcN)(2) as the major product. The unique enzymatic properties of this chitosanase may make it a good candidate for (GlcN)(2) production.

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