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Identification of sea cucumber species in processed food products by PCR-RFLP method

文献类型： 外文期刊

作者： Zeng, Ling 1 ; Wen, Jing 2 ; Fan, Sigang 3 ; Chen, Ziming 1 ; Xu, Youhou 4 ; Sun, Yulin 2 ; Chen, Daohai 2 ; Zhao, Juan 2 ;

作者机构： 1.Lingnan Normal Univ, Dept Chem, Zhanjiang 524048, Peoples R China

2.Lingnan Normal Univ, Dept Biol, Zhanjiang 524048, Peoples R China

3.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab Sout China Sea Fishery Resources Utilizat, Minist Agr, Guangzhou 510300, Guangdong, Peoples R China

4.Qinzhou Univ, Guangxi Key Lab Beibu Gulf Marine Biodivers Conse, Qinzhou 535000, Peoples R China

关键词： Sea cucumbers; Species identification; 16S rRNA gene; PCR-RFLP; FINS

期刊名称：FOOD CONTROL （ 影响因子：5.548； 五年影响因子：5.498 ）

ISSN： 0956-7135

年卷期： 2018 年 90 卷

页码：

收录情况： SCI

摘要： A PCR-RFLP method has been developed for the identification of 16 commercial sea cucumber species in raw and processed food products. To implement the method, PCR amplification with the 16Sar/16Sbr primers, targeted to the amplification of a ca. 570 bp region of the 16S rRNA mitochondrial gene in sea cucumbers, was coupled to restriction analysis with Dde I, Hae Ill and Sty I. We also report the FINS method based on 16S rRNA mitochondrial sequences. Both proposed methodologies were independently applied to authenticate the species of 19 commercial products, showing that 9 products were incorrectly labeled (48%). Therefore, the 16S rRNA mitochondrial gene really provide convenient, useful and academic molecular marker to study questions related to correct labeling of commercial products, traceability, and the control of sea cucumber fisheries. (C) 2018 Elsevier Ltd. All rights reserved.

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