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Assessment of myoblast circular RNA dynamics and its correlation with miRNA during myogenic differentiation

文献类型： 外文期刊

作者： Zhang, Pengpeng 1 ; Xu, Haixia 1 ; Li, Rui 1 ; Wu, Wei 1 ; Chao, Zhe 2 ; Li, Cencen 1 ; Xia, Wei 3 ; Wang, Lei 1 ; Yang, Jin 1 ;

作者机构： 1.Xinyang Normal Univ, Coll Life Sci, Xinyang 464000, Peoples R China

2.Hainan Acad Agr Sci, Inst Anim Sci & Vet Med, Haikou 571100, Hainan, Peoples R China

3.Southwest Univ Nationalities, Sichuan Key Lab Conservat & Utilizat Anim Genet R, Chengdu 610041, Sichuan, Peoples R China

4.Univ Hawaii Manoa, Dept Human Nutr Food & Anim Sci, Honolulu, HI 96822 USA

关键词： Circular RNA; Myoblast; Myogenesis; High-throughput RNA sequencing; microRNA sponges

期刊名称：INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY （ 影响因子：5.085； 五年影响因子：4.407 ）

ISSN： 1357-2725

年卷期： 2018 年 99 卷

页码：

收录情况： SCI

摘要： Myoblast differentiation is a highly complex process that is regulated by proteins as well as by non-coding RNAs. Circular RNAs have been identified as an emerging new class of non-coding RNA in the modulation of skeletal muscle development, whereas their expression profiles and functional regulation in myoblast differentiation remain unknown. In the present study, we performed deep RNA-sequencing of C2C12 myoblasts during cell differentiation and uncovered 37,751 unique circular RNAs derived from 6943 hosting genes. The ensuing qRT-PCR and RNA fluorescence in situ hybridization verification were carried out to confirm the RNA-sequencing results. An unbiased analysis demonstrated dynamic circular RNA expression changes in the process of myoblast differentiation, and the circular RNA abundances were independent from their cognate linear RNAs. Gene ontology analysis showed that many down-regulated circular RNAs were exclusive to cell division and the cell cycle, whereas up-regulated circular RNAs were related to the cell development process. Furthermore, interaction networks of circular RNA-microRNA were constructed. Several microRNAs well-known for myoblast regulation, such as miR-133, miR-24 and miR-23a, were in this network. In summary, this study showed that circular RNA expression dynamics changed during myoblast differentiation. Circular RNAs play a role in regulating the myoblast cell cycle and development by acting as microRNA binding sites to facilitate their regulation of gene expression during myoblast differentiation. These findings open a new avenue for future investigation of this emerging RNA class in skeletal muscle growth and development.

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