Genome-Wide Identification and Functional Prediction of Novel Drought-Responsive lncRNAs in Pyrus betulifolia
文献类型： 外文期刊
作者： Wang, Jinxing 1 ; Lin, Jing 1 ; Kan, Jialiang 1 ; Wang, Hong 1 ; Li, Xiaogang 1 ; Yang, Qingsong 1 ; Li, Hui 1 ; Chang, Y 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Pomol, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 200014, Jiangsu, Peoples R China
关键词： Pyrus betulifolia; drought stress; lncRNA; identification
期刊名称：GENES （ 影响因子：4.096； 五年影响因子：4.339 ）
ISSN： 2073-4425
年卷期： 2018 年 9 卷 6 期
页码：
收录情况： SCI
摘要： Increasing evidence shows that long noncoding RNAs (lncRNAs) play important roles in developmental regulation and many other biological processes in plants. However, identification of lncRNAs in Pyrus betulifolia is limited compared with studies of functional gene expression. Using high-throughput sequencing technology, the transcriptome of P. betulifolia under drought stress was analyzed to identify lncRNAs. A total of 14,478 lncRNAs were identified, of which 251 were found to be drought-responsive. The putative target genes of these differentially expressed lncRNAs were significantly enriched in metabolic processes, organic substance metabolic processes, macromolecule metabolic processes, and heterocyclic compound binding. Real-time quantitative polymerase chain reaction validation suggested that the results of the RNA sequencing data analysis were reliable. This study will provide genetic resources for pear breeding and provide reference to other pomological studies.
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