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A Broad-Spectrum Phage Endolysin (LysCP28) Able to Remove Biofilms and Inactivate Clostridium perfringens Strains

文献类型： 外文期刊

作者： Lu, Rui 1 ; Liu, Banhong 2 ; Wu, Liting 2 ; Bao, Hongduo 2 ; Garcia, Pilar 4 ; Wang, Yongjuan 5 ; Zhou, Yan 2 ; Zhang, Hui 1 ;

作者机构： 1.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Peoples R China

2.Jiangsu Acad Agr Sci, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base MOST, Nanjing 210014, Peoples R China

3.Shaanxi Univ Sci & Technol, Sch Food & Biol Engn, Xian 710021, Peoples R China

4.CSIC, Dairy Res Inst Asturias IPLA, Villaviciosa 33300, Spain

5.Jiangsu Agrianim Husb Vocat Coll, Taizhou 225300, Peoples R China

关键词： Clostridium perfringens; phage; endolysin; biofilm; antimicrobial; duck meat

期刊名称：FOODS （ 影响因子：5.2； 五年影响因子：5.5 ）

ISSN：

年卷期： 2023 年 12 卷 2 期

页码：

收录情况： SCI

摘要： Clostridium perfringens is a gram-positive, anaerobic, spore-forming bacterium capable of producing four major toxins which cause disease symptoms and pathogenesis in humans and animals. C. perfringens strains carrying enterotoxins can cause food poisoning in humans and are associated with meat consumption. An endolysin, named LysCP28, is encoded by orf28 from C. perfringens bacteriophage BG3P. This protein has an N-terminal glycosyl-hydrolase domain (lysozyme) and a C-terminal SH3 domain. Purified LysCP28 (38.8 kDa) exhibited a broad spectrum of lytic activity against C. perfringens strains (77 of 96 or 80.21%), including A, B, C, and D types, isolated from different sources. Moreover, LysCP28 (10 mu g/mL) showed high antimicrobial activity and was able to lyse 2 x 10(7) CFU/mL C. perfringens ATCC 13124 and C. perfringens J21 (animal origin) within 2 h. Necessary due to this pathogenic bacterium's ability to form biofilms, LysCP28 (18.7 mu g/mL) was successfully evaluated as an antibiofilm agent in both biofilm removal and formation inhibition. Finally, to confirm the efficacy of LysCP28 in a food matrix, duck meat was contaminated with C. perfringens and treated with endolysin (100 mu g/mL and 50 mu g/mL), which reduced viable bacteria by 3.2 and 3.08 units-log, respectively, in 48 h at 4 degrees C. Overall, the endolysin LysCP28 could potentially be used as a biopreservative to reduce C. perfringens contamination during food processing.

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