A novel method to detect meat adulteration by recombinase polymerase amplification and SYBR green I
文献类型： 外文期刊
作者： Cao, Yuhao 1 ; Zheng, Kaizhi 2 ; Jiang, Junfang 2 ; Wu, Jianliang 2 ; Shi, Fangxiong 1 ; Song, Xuemei 2 ; Jiang, Yongq 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China
2.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet, Hangzhou 310021, Zhejiang, Peoples R China
关键词： Meat adulteration; RPA; Species identification; SYBR green I; Visualize
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2018 年 266 卷
页码：
收录情况： SCI
摘要： Meat adulteration is one of the most common economic fraudulences in food industry. Current methodologies of meat source identification are complex, time-consuming and require sophisticated equipment. Hence, a simpler species specific method to determinate species is urgently needed. Here, we developed a novel method to visually identify the adulteration of meat using recombinase polymerase amplification (RPA) and SYBR green I (SG). At the isothermal temperature of 37 degrees C, RPA specifically identifies duck, chicken, cow, sheep and pig within 30 min of water bath. The RPA amplicons were successfully visualized by adding SG I. Furthermore, RPA can differentiate species of boiled, microwaved, high pressured or fried samples. Finally, using this system, we visually identified 1% pork adulterated in mutton or beef.
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