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Adsorptive immobilization of agro-industrially produced crude laccase on various micro-biochars and degradation of diclofenac

文献类型： 外文期刊

作者： Lonappan, Linson 1 ; Liu, Yuxue 1 ; Rouissi, Tarek 1 ; Brar, Satinder Kaur 1 ; Verma, Mausam 3 ; Surampalli, Rao Y. 4 ;

作者机构： 1.Univ Quebec, INRS ETE, 490 Rue Couronne, Quebec City, PQ G1K 9A9, Canada

2.Zhejiang Acad Agr Sci, Inst Environm Resources Soil & Fertilizer, 198 Shiqiao Rd, Hangzhou 310021, Zhejiang, Peoples R China

3.CO2 Solut Inc, 2300 Rue Jean Perrin, Quebec City, PQ G2C 1T9, Canada

4.Univ Nebraska Lincoln, Dept Civil Engn, N104 SEC,POB 886105, Lincoln, NE 68588 USA

关键词： Laccase; Biochar; Biotransformation; Adsorptive immobilization; Isotherms; Desorption

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：7.963； 五年影响因子：7.842 ）

ISSN： 0048-9697

年卷期： 2018 年 640 卷

页码：

收录情况： SCI

摘要： Although enzymes are gifted with unique and unprecedented catalytic activity and selectivity over a wide range of pollutants, still their stability related issues often hinder their application in real environmental conditions. In this study, agro-industrially produced crude laccase was concentrated using ultrafiltration. Crude laccase was immobilized on pine wood (BC-PW), pigmanure (BC-PM) and almond shell (BC-AS) biochar microparticles. Immobilization of laccase was investigated at various laccase activities on microbiochars and the release (desorption) of the enzyme has been studied. It was observed that for all the biochars, as the initial concentration of laccase increased in the crude solution, the binding capacity and as result immobilization efficiency also increased. BC-PM was found to be the most effective (31.4 +/- 3.1 U g(-1)) at 10 U mL(-1) of enzyme activity followed by BC-AS (24.3 +/- 4.8 U g(-1)) and BC-PW(14.58 +/- 3.3 U g(-1)). In addition, the biochars were functionalized with citric acid for possible surface modifications and the effect of biochars for the adsorption of enzymes has been investigated. Isotherm studies of enzyme loading onto biochar established homogeneous monolayer adsorption as the major mechanism. The desorption of laccase from all biochars followed pseudo-second-order model. Immobilized laccase exhibited superior storage ability and shelf-life which were three times higher than free laccase. Finally, the immobilized laccase was used for the degradation of micropollutant, DCF and near 100% removal was obtained within 5 h at an environmentally relevant concentration (500 mu g L-1). (c) 2018 Elsevier B.V. All rights reserved.

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