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QTL Mapping of Fiber-Related Traits Based on a High-Density Genetic Map in Flax (Linum usitatissimum L.)

文献类型： 外文期刊

作者： Wu, Jianzhong 1 ; Zhao, Qian 3 ; Zhang, Liyan 3 ; Li, Suiyan 1 ; Ma, Yanhua 1 ; Pan, Liyan 1 ; Lin, Hong 1 ; Wu, Guangwen 3 ;

作者机构： 1.Heilongjiang Acad Agr Sci, Inst Forage & Grassland Sci, Harbin, Heilongjiang, Peoples R China

2.Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin, Peoples R China

3.Heilongjiang Acad Agr Sci, Harbin, Heilongjiang, Peoples R China

4.Heilongjiang Aca

关键词： Linum usitatissimum L.; specific-locus amplified fragment (SLAF); high-density genetic map; quantitative trait loci (QTL); flax

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.753； 五年影响因子：6.612 ）

ISSN： 1664-462X

年卷期： 2018 年 9 卷

页码：

收录情况： SCI

摘要： A geneticmap is an important and valuable tool for quantitative trait locus (QTL) mapping, marker-assisted selection (MAS)-based breeding, and reference-assisted chromosome assembly. In this study, 112 F-2 plants from a cross between Linum usitatissimum L. "DIANE" and "NY17" and parent plants were subjected to high-throughput sequencing and specific-locus amplified fragment (SLAF) library construction. After preprocessing, 61.64 Gb of raw data containing 253.71Mb paired-end reads, each 101 bp in length, were obtained. A total of 192,797 SLAFs were identified, of which 23,115 were polymorphic, with a polymorphism rate of 11.99%. Finally, 2,339 SLAFs were organized into a linkage map consisting of 15 linkage groups (LGs). The total length of the genetic map was 1483.25 centimorgans (cM) and the average distance between adjacent markers was 0.63 cM. Combined with flax chromosome-scale pseudomolecules, 12 QTLs associating with 6 flax fiber-related traits were mapped on the chromosomal scaffolds. This high-density genetic map of flax should serve as a foundation for flax fine QTL mapping, draft genome assembly, and MAS-guided breeding. Ultimately, the genomic regions identified in this research could potentially be valuable for improving flax fiber cultivars, as well as for identification of candidate genes involved in flax fiber formation processes. SIGNIFICANCE STATEMENT A high-density genetic map of flax was constructed, and QTLs were identified on the sequence scaffolds to be interrelated with fiber-related traits. The results of this study will not only provide a platform for gene/QTL fine mapping, map-based gene isolation, and molecular breeding for flax, but also provide a reference to help position sequence scaffolds on the physical map and assist in the process of assembling the flax genome sequence.

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