文献类型： 外文期刊

作者： Li, Yin-Xia ¹ ; Gao, Yi-Long ³ ; He, Xing-Liang ³ ; Cao, Shao-Xian ¹ ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing, Jiangsu, Peoples R China

2.Jiangsu Acad Agr Sci, Key Lab Anim Breeding & Reprod, Nanjing, Jiangsu, Peoples R China

3.Minist Publ Secur, Nanjing Policedog Res Inst, Policedog Technol Key Lab, Nanjing, Jiangsu, Peoples R China

关键词： Tibetan Mastiff; mitochondrial control region; hypervariable region; genetic diversity

期刊名称：MITOCHONDRIAL DNA PART A （ 影响因子：1.514； 五年影响因子：0.707 ）

ISSN： 2470-1394

年卷期： 2018 年 29 卷 5 期

页码：

收录情况： SCI

摘要： The control region of mitochondrial DNA (mtDNA) was obtained from 40 purebred Chinese Tibetan Mastiffs (TMs). Sequence structure and genetic diversity were analyzed, and a phylogenetic tree was constructed. The TM mtDNA control region was composed of ETAS (extended termination associated sequences), CD (a central domain) and CSBs (conserved sequenced blocks) and sequence length showed some diversity, which was mainly caused by the number of 10 nucleotide repeat units [5'-GTA CAC GT (G/A) C-3'] between CSB I and CSB II, which ranged from 27 to 35 among individuals. Seventy-five polymorphic sites were identified, which defined 37 haplotypes; the haplotype diversity was 0.990, and the nucleotide diversity was 1.201. Based on the control region sequences, Chinese TMs were divided into three categories, which were consistent with the origin and geographical classification of TMs. Phylogenetic analysis of 538-bp HVR-I sequences revealed that TMs were most closely related to Labrador Retrievers.