文献类型： 外文期刊

作者： Wang, Shui-Lian ¹ ; Han, Li ² ; Ahmad, S. ² ; Cao, Shao-Xian ⁴ ; Xue, Li-Qun ¹ ; Xing, Zhao-Fang ⁵ ; Feng, Jian-Zhong; ¹ ;

作者机构： 1.Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China

2.Huazhong Agr Univ, Coll Anim Sci & Technol, Wuhan 430070, Peoples R China

3.Univ Agr Faisalabad, Dept Livestock Management, Faisalabad 38040, Pakistan

4.Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing 210041, Jiangsu, Peoples R China

5.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China

6.Tianjin Zhongyi Anim & Sci Technol Ltd Co, Tianjin 300457, Peoples R China

期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The objective was to investigate the effects of a novel DNA vaccine (pcISI) harboring two copies of inhibin alpha (1-32) fragments on immune response, hormone concentrations and reproductive performance in rats. Female Wistar rats (n = 18 per group) were immunized (twice, 4 wk apart) with 10, 50, or 100 mu g (T-1, T-2 and T-3, respectively), of the pcISI plasmid. At 4 wk after the second immunization, plasma antibody titers were higher (P < 0.05) in T-3 than in either T-1 or T-2 (0.341 +/- 0.123, 0.236 +/- 0.068, and 0.251 +/- 0.077, respectively, mean +/- SD). Concurrrently, plasma concentrations of FSH and estradiol were highest (P < 0.05) in T-3, and were higher (P < 0.05) in T-1 and T-2 than in control groups. For antibody-positive rats, there was a correlation (P < 0.01) between antibody titer and FSH concentrations after two pcISI immunizations. The number of mature follicles in the T-3 group (46.00 +/- 4.65) was higher (P < 0.05) than in two control groups (29.25 +/- 3.72 and 27.92 +/- 3.48), and also higher (P < 0.05) than in T-1 and T-2 (37.17 +/- 4.99 and 38.75 +/- 7.09). Antibody-positive rats had more mature ovarian follicles than negative rats (46.75 +/- 4.23 vs. 35.60 +/- 3.38, P < 0.05). Moreover, litter size and number of placentas were increased (P < 0.05) in the pcISI immunization groups, except for the T-1 group, compared to the control groups. In conclusion, the pcISI DNA vaccine successfully induced a humoral immune response, improved reproductive hormone concentrations, stimulated follicular development, and increased number of placentas and litter size. Furthermore, 100 mu g yielded the best immune response. (c) 2012 Elsevier Inc. All rights reserved.