文献类型： 外文期刊

作者： Zhang, Fengxia ¹ ; Chen, Siqian ¹ ; Yang, Ting ¹ ; Ao, Hong ³ ; Zhai, Liwei ¹ ; Li, Qianjun ² ; Xing, Kai ⁴ ; Liu, Yibing ¹ ;

作者机构： 1.China Agr Univ, Dept Anim Genet & Breeding, Natl Engn Lab Anim Breeding, MOA Key Lab Anim Genet & Breeding, Beijing, Peoples R China

2.Tianjin Acad Agr Sci, Inst Anim Husb & Vet, Innovat Team Pig Hlth Breeding, Tianjin, Peoples R China

3.Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing, Peoples R China

4.Beijing Univ Agr, Anim Sci & Technol Coll, Beijing, Peoples R China

关键词： Pig; PRRSV-specific antibody; DNA methylation; Intergenerational transmission

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2021 年 801 卷

页码：

收录情况： SCI

摘要： The main strategy for preventing porcine reproductive and respiratory syndrome (PRRS) is vaccination. However, current commercial porcine reproductive and respiratory syndrome virus (PRRSV) vaccines have limited effectiveness and may even cause infections in pigs. The identification of stable molecular markers associated with immune responses to PRRSV vaccination in pigs provides a new approach for PRRS prevention. DNA methylation, the most stable epigenetic molecular marker related to PRRSV vaccination, has not been investigated. In the current research, we used whole genome bisulfite sequencing (WGBS) to investigate DNA methylation in pregnant sows that received PRRSV vaccination and their piglets with high and low PRRSVspecific antibody levels. By performing methylation data analysis and basing on our previous transcriptomic studies, we identified several differentially methylated genes (DMGs) that are involved in the pathways of inflammatory and immune responses. Among the DMGs, ISG15, MX1, SERPINE1, GNG11 and IFIT3 were common hub genes in the two generations. MX1 and GNG11 were located in quantitative trait loci related with PRRSV antibody titer and PRRSV susceptibility, respectively. These results suggest that PRRSV vaccination in sows induces DNA methylation changes in genes and DNA methylation changes occur through intergenerational transmission. The novel DNA methylation markers and target genes observed in our study provide new insights into the molecular mechanisms of immune responses to PRRSV vaccination across two pig generations.