文献类型： 外文期刊

作者： Liu, Chuncheng ¹ ; Chen, Min ² ; Wang, Meng ² ; Pi, Wenhui ⁴ ; Lie, Ning ² ; Meng, Qingyong ¹ ;

作者机构： 1.China Agr Univ, Coll Biol Sci, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Beijing, Peoples R China

2.China Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, Beijing, Peoples R China

3.China Agr Univ, Coll Anim Sci & Technol, Beijing, Peoples R China

4.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi, Peoples R China

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2018 年 13 卷 7 期

页码：

收录情况： SCI

摘要： MiRNAs play an important role in cell proliferation, apoptosis, and differentiation. MiR-18a is increasingly being recognized as a regulator of cancer pathogenesis. Here, we discovered that miR-18a participates in myoblasts proliferation. Expression of miR-18a was downregulated with the differentiation of C2C12 myoblasts. Overexpression of miR-18a affected the proliferation of C2C12 cells, primary myoblasts and RD cells. MiR-18a influenced the expression of cell cycle-related genes. Using TargetScan 6.2, we found that the 3' untranslated region (UTR) of the mouse Fgfl gene contains complementary sequences to miR18a. Using a siRNA, we confirmed that the reduction in the Fgf1 levels inhibited proliferation of C2C12 cells. Therefore, our results show that miR-18a participates in the regulation of proliferation by partly decreasing the expression of Fgf1.