Optimization of the process parameters for reduction of gossypol levels in cottonseed meal by functional recombinant NADPH-cytochrome P450 reductase and cytochrome P450 CYP9A12 of Helicoverpa armigera
文献类型： 外文期刊
作者： Chen, Cheng 1 ; Zhang, Yan 2 ; Pi, Wenhui 3 ; Yang, Wenting 1 ; Nie, Cunxi 1 ; Liang, Jing 1 ; Ma, Xi 1 ; Zhang, Wen-Ju 1 ;
作者机构： 1.Shihezi Univ, Coll Anim Sci & Technol, Shihezi 832000, Xinjiang, Peoples R China
2.Shihezi Univ, Sch Chem & Chem Engn, Shihezi 832000, Xinjiang, Peoples R China
3.Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi 832000, Xinjiang, Peoples R China
4.China Agr Univ, Coll Anim Sci & Technol, State Key Lab Anim Nutr, Beijing 100193, Peoples R China
关键词： Helicoverpa armigera; Cytochrome P450; CYP9A12; Gossypol; Detoxification
期刊名称：AMB EXPRESS （ 影响因子：3.298； 五年影响因子：3.427 ）
ISSN： 2191-0855
年卷期： 2019 年 9 卷
页码：
收录情况： SCI
摘要： Gossypol is a toxic polyphenolic product that is derived from cotton plants. The toxicity of gossypol has limited the utilization of cottonseed meal (CSM) in the feed industry. The gene, Helicoverpa armigera CYP9A12, is a gossypol-inducible cytochrome P450 gene. The objective of our study was to obtain the functional recombinant H. armigera CYP9A12 enzyme in Pichia pastoris and to verify whether this candidate enzyme could decrease gossypol in vitro. Free and total gossypol contents were detected in the enzyme solution and in CSM. The H. armigera CYP9A12 enzyme degraded free concentration of gossypol. After optimization of the single-test and response surface method, free gossypol content could be decreased to 40.91 mg/kg in CSM by the H. armigera CYP9A12 enzyme when the initial temperature was 35 degrees C, the enzymatic hydrolysis time lasted 2.5 h, the enzyme addition was 2.5 mL, and the substrate moisture was 39%.
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