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Application of the red fluorescent protein mCherry in mycelial labeling and organelle tracing in the dermatophyte Trichophyton mentagrophytes

文献类型： 外文期刊

作者： Xiao, Chenwen 1 ; Li, Ling 2 ; Lao, Limin 4 ; Liu, Yan 1 ; Wei, Qiang 1 ; Ji, Quan'an 1 ; Sun, Guochang 2 ; Lin, Fucheng; 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Zhejiang, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, Hangzhou 310021, Zhejiang, Peoples R China

3.Zhejiang Agr & Forest Univ, Sch Agr & Food Sci, Hangzhou 311300, Zhejiang, Peoples R China

4.Zhejiang Univ, Affiliated Hosp 2, Dept Dermatol, Coll Med, Hangzhou 310009, Zhejiang, Peoples R China

5.Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou,

关键词： Trichophyton mentagrophytes; fluorescent; mCherry; AtMT

期刊名称：FEMS MICROBIOLOGY LETTERS （ 影响因子：2.742； 五年影响因子：2.856 ）

ISSN： 0378-1097

年卷期： 2018 年 365 卷 6 期

页码：

收录情况： SCI

摘要： Trichophyton mentagrophytes is a fungus that causes skin disease in humans and other animals worldwide. Studies on molecular biology and fluorescent labeling of the fungus are limited. Here, we applied mCherry for the first time in T. mentagrophytes to label the fungus and its organelles. We constructed four expression vectors of mCherry or mCherry fusions containing a variety of resistance markers and promoters, which were then integrated, together with two previous mCherry expression vectors, in T. mentagrophytes via Agrobacterium tumefaciens-mediated transformation (AtMT). The resulting transformants emitted bright red fluorescence. We used the histone protein H2B and the peroxisome targeting signal 1 (PTS1) peptide to target the nucleus and peroxisomes, respectively, in T. mentagrophytes. In the transformants expressing mCherry-fused H2B, the fluorescence was distinctly localized to the nuclei in hyphae, spores and the fungal cells in infected animal tissue. In the T. mentagrophytes transformants where the peroxisome was targeted, the mCherry was present as small dots (0.2-1 mu m diameter) throughout the spores and the hyphae. We also constructed a T. mentagrophytes AtMT library containing more than 1000 hygromycin-resistant transformants that were genetically stable. Our results provide useful tools for further investigations on molecular pathogenesis of T. mentagrophytes.

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