Optimization extraction, characterization and anticancer activities of polysaccharides from mango pomace
文献类型： 外文期刊
作者： Hu, Huigang 1 ; Zhao, Qiaoli 1 ; Pang, Zhencai 1 ; Xie, Jianghui 3 ; Lin, Lijing 2 ; Yao, Quansheng 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, South Subtrop Crops Res Inst, Minist Agr, Key Lab Trop Fruit Tree Biol, Zhanjiang 524091, Peoples R China
2.Chinese Acad Trop Agr Sci, Agr Prod Proc Res Inst, Zhanjiang 524001, Peoples R China
3.Chinese Acad Trop Agr Sci, Haikou 571101, Hainan, Peoples R China
关键词： Mango pomace; Polysaccharides; Ultrasonic-assisted extraction; Characterization; Anticancer activity; Response surface methodology
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2018 年 117 卷
页码：
收录情况： SCI
摘要： Response surface methodology was used to optimize the extraction conditions for ultrasonic-assisted extraction of polysaccharides from mango pomace. The Optimum extraction conditions consisted of extraction temperature of 74 degrees C, ultrasonic power of 170 W, extraction time of 100 min, and raw material-to-water ratio of 1:40 g/mL Under these conditions, the extraction yield was 3.71 +/- 0.07%. Three novel polysaccharide fractions, MG-1, MG-2 and MG-3 were purified from the crude polysaccharides by using DEAE-52 cellulose and Sephadex G-100 column chromatography. The molecular weight and monosaccharide composition of polysaccharide fractions (MPFs) were analyzed by high performance liquid gel permeation chromatography (HPGPC) and HPLC analysis, respectively. The characterizations of MPFs were conducted with FT-IR, H-1 NMR and SEM. Furthermore, the anticancer activities of the polysaccharide fractions were also investigated in vitro. Results showed that MG-1, MG-2 and MG-3 exhibited significant anticancer activities against HepG2, MCF-7, A549, HeLa, A2780, HCT-116 and BGC-823 cells in a dose-dependent manner. MPFs were also showed to promote apoptosis as seen in the nuclear morphological examination study using calcein acetyl methoxy methyl easter (calcein-AM) and propidium iodide (PI) staining. This research could serve as a theoretical reference for the efficient utilization of MPFs in biomedical and functional food. (C) 2018 Elsevier B.V. All rights reserved.
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