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Label-free fluorescent aptasensor berberine-based strategy for ultrasensitive detection of Hg2+ ion

文献类型： 外文期刊

作者： Song, Xiuli 1 ; Fu, Baochun 3 ; Lan, Yifeng 2 ; Chen, Yanxia 2 ; Wei, Yanli 2 ; Dong, Chuan 2 ;

作者机构： 1.Taiyuan Normal Univ, Dept Chem, Jinzhong 030619, Peoples R China

2.Shanxi Univ, Inst Environm Sci, Taiyuan 030006, Shanxi, Peoples R China

3.Shanxi Acad Agr Sci, Inst Hort, Taiyuan 030031, Shanxi, Peoples R China

关键词： Berberine; Aptasensor; Hg2+ ion; Fluorescence; Label-free

期刊名称：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY （ 影响因子：4.098； 五年影响因子：3.464 ）

ISSN： 1386-1425

年卷期： 2018 年 204 卷

页码：

收录情况： SCI

摘要： A label-free fluorescent aptasensing platform was fabricated and a simple and rapid method to detect Hg2+ ion in aqueous solution was put forward by means of berberine and Hg2+ ion-aptamer are as the fluorescence probe and the recognition element, respectively. Various factors including the concentration of berberine, Hg2+ ion and Hg2+ ion-aptamer, pH effect and the reaction time were investigated in detail. Under the optimal experimental conditions, in the sensing system, the fluorescence intensity changes displayed a calibration response for Hg2+ ion in the range of 0.1 mu M to 10.0 mu M and the detection limit was of 7.7 nM (S/N = 3). The fabricated label-free fluorescence aptasensor is not only conveniently but also effectively applicable used for analysis of Hg2+ ion in blood serum and tap water samples and the recovery range is of 96.0%-105.7%. In brief, this study offers an easy, economical and stable assay system for detecting Hg2+ ion in rough condition. (C) 2018 Elsevier B.V. All rights reserved.

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