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Highly sensitive analysis of tetrodotoxin based on free-label fluorescence aptamer sensing system

文献类型： 外文期刊

作者： Lan, Yifeng 1 ; Qin, Guojie 2 ; Wei, Yanli 1 ; Dong, Chuan 1 ; Wang, Li 1 ;

作者机构： 1.Shanxi Univ, Inst Environm Sci, Taiyuan 030006, Shanxi, Peoples R China

2.Shanxi Acad Agr Sci, Inst Hort, Taiyuan 030031, Shanxi, Peoples R China

关键词： Tetrodotoxin; Label-free; Berberine; Fluorescence; Aptamer sensor

期刊名称：SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY （ 影响因子：4.098； 五年影响因子：3.464 ）

ISSN： 1386-1425

年卷期： 2019 年 219 卷

页码：

收录情况： SCI

摘要： Tetrodotoxin (TTX) specifically can bind to its nucleic add aptamer (TTX-aptamer) and cause the conformation of TD(-aptamer to be switched from the single-strand random coiling form to the compact neck ring structure. Based on the microenvironment difference of the fluorescence reporter, berberine in between the single-stranded coil oligonucleotides and the structure of the neck ring, a simple, rapid and sensitive label-free fluorescence aptamer sensing system for detection of TTX was developed. Various factors affecting the analysis of TTX were optimized, including the concentration of berberine, ion strength, pH, reaction time, the concentration of TTX-aptamer. Under the optimal experimental conditions, the fluorescence intensity of the sensing system and the concentration of TTX showed a good linear relationship in the range of 0.1 nM to 500 nM, with the detection limit of 0.074 nM. The standard recovery test result exhibited that the recoveries of TIX in serum samples were 96.54%-106.40%. The established method has the advantages of high specificity, good sensitivity, quickness and convenience, low cost, and can be used for the detection of TTX in serum samples. (C) 2019 Elsevier B.V. All rights reserved.

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