In vitro and in silico perspectives on estrogenicity of tanshinones from Salvia miltiorrhiza
文献类型： 外文期刊
作者： Zhang, Tiehua 1 ; Zhong, Shuning 1 ; Wang, Yongjun 2 ; Dong, Shuyue 1 ; Guan, Tianzhu 1 ; Hou, Ligang 2 ; Xing, XiaoJi 1 ;
作者机构： 1.Jilin Univ, Coll Food Sci & Engn, Changchun 130062, Jilin, Peoples R China
2.Jilin Acad Agr Sci, Inst Agr Resources & Environm, Changchun 130033, Jilin, Peoples R China
关键词： Estrogenic activity; Tanshinones; Fluorescence polarization; Luciferase reporter assay; Molecular docking
期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）
ISSN： 0308-8146
年卷期： 2019 年 270 卷
页码：
收录情况： SCI
摘要： This work aims to investigate the structure-activity relationship for binding and activation of human estrogen receptor alpha ligand binding domain (hER alpha-LBD) with tanshinones by a combination of in vitro and in silico approaches. The recombinant hER alpha-LBD was expressed in E. coli strain. The direct binding interactions of tanshinones with hER alpha-LBD and their ER alpha agonistic potency were investigated by fluorescence polarization (FP) and reporter gene assays, respectively. FP assay suggested that the tested tanshinones can bind to hER alpha-LBD as affinity ligands. Tanshinones acted as agonists of hER alpha as demonstrated by transactivation of estrogen response element (ERE) in transiently transfected MCF-7 cells and by molecular docking of these compounds into the hydrophobic binding pocket of hER alpha-LBD. Interestingly, comparison of the calculated binding energies versus Connolly solvent-excluded volume and experimental binding affinities showed a good correlation. This work may provide insight into chemical and pharmacological characterization of novel bioactive compounds from Salvia miltiorrhiza.
- 相关文献
作者其他论文 更多>>
-
Increased topsoil depth required to support increased grain yield production in high density maize
作者：Zhang, Xiaolong;Kong, Yuanyuan;Shao, Xiwen;Geng, Yanqiu;Wang, Lichun;Wang, Yongjun;Zhang, Xiaolong;Kong, Yuanyuan;Lv, Yanjie;Yao, Fanyun;Cao, Yujun;Wang, Lichun;Wang, Yongjun
关键词：Grain yield; Harvest index; Root characteristics; Leaf area index; Net photosynthetic rate
-
Tillage effects on residue-derived carbon distribution among soil fractions in a Mollisol
作者：Zhang, Yan;Liang, Aizhen;Huang, Dandan;Zhang, Shaoqing;Zhang, Yang;Gao, Yan;Chen, Xuewen;Zhang, Shixiu;Gregorich, Edward G.;McLaughlin, Neil B.;Guo, Yafei;Wang, Yongjun;Zhang, Yang
关键词：Residue-derived carbon; Tillage practices; Physical and chemical fractions
-
Research Progress and Development Trends of Greenhouse Gas Emissions from Cereal-Legume Intercropping Systems
作者：Yao, Fanyun;Liu, Xiaodan;Cao, Yujun;Lv, Yanjie;Wei, Wenwen;Xu, Wenhua;Liu, Zhiming;Liang, Jie;Wang, Yongjun;Wu, Yang
关键词：bibliometric analysis; soil greenhouse gas emissions; crop residue retention; nitrogen fertilizer; tillage
-
Revolutionizing Maize Crop Productivity: The Winning Combination of Zigzag Planting and Deep Nitrogen Fertilization for Maximum Yield through Root-Shoot Ratio Management
作者：Zheng, Yongzhao;Yue, Yang;Ren, Hong;Gong, Xiangwei;Jiang, Ying;Qi, Hua;Zheng, Yongzhao;Yue, Yang;Li, Congfeng;Wang, Yongjun;Zhang, Hongyu
关键词：canopy structure; grain yield; nutrients; plant and row spacing; root morphology; leaf morphology; spring maize (Zea mays L.,)
-
A simple method for lodging resistance evaluation of maize in the field
作者：Yang, Jinsheng;Meng, Xiangzeng;Yang, Qinglong;Zheng, Peifeng;Shao, Xiwen;Wang, Yongjun;Wang, Lichun;Yang, Jinsheng;Wang, Yongjun;Wang, Lichun;Yang, Shuangyuan;Yang, Jinzhong;Li, Zhaoxia
关键词：maize; lodging resistance; tester; nondestructive evaluation; in situ; actual measured value; presumptive value
-
Estimation of maize straw production and appropriate straw return rate in China
作者：Liu, Wanmao;Liu, Guangzhou;Xie, Ruizhi;Ming, Bo;Wang, Keru;Xue, Jun;Li, Shaokun;Hou, Peng;Liu, Wanmao;Liu, Yuee;Yang, Yunshan;Guo, Xiaoxia;Wang, Yonghong;Zhao, Rulang;Zhang, Wenjie;Wang, Yongjun;Bian, Shaofeng;Ren, Hong;Zhao, Xiaoyan;Liu, Peng;Chang, Jianzhi;Zhang, Guohe;Liu, Jiayou;Yuan, Liuzheng;Zhao, Haiyan;Shi, Lei;Zhang, Lili;Yu, Lin;Gao, Julin;Yu, Xiaofang;Wang, Zhigang;Shen, Liguo;Ji, Ping;Yang, Shuzong;Zhang, Zhongdong;Xue, Jiquan;Wang, Xiuquan;Wang, Xiuquan;Lu, Tingqi;Dong, Benchun;Li, Gang;Ma, Baoxin;Li, Jinqin;Deng, Xiufeng;Liu, Yonghong;Yang, Qin;Jia, Chunlan;Chen, Xianping;Fu, Hua
关键词：Maize straw production; Straw return; Carbon input; Carbon emission; Soil carbon sequestration
-
Estimation of maize straw production and appropriate straw return rate in China
作者：Liu, Wanmao;Liu, Guangzhou;Xie, Ruizhi;Ming, Bo;Wang, Keru;Xue, Jun;Li, Shaokun;Hou, Peng;Liu, Wanmao;Liu, Yuee;Yang, Yunshan;Guo, Xiaoxia;Wang, Yonghong;Zhao, Rulang;Zhang, Wenjie;Wang, Yongjun;Bian, Shaofeng;Ren, Hong;Zhao, Xiaoyan;Liu, Peng;Chang, Jianzhi;Zhang, Guohe;Liu, Jiayou;Yuan, Liuzheng;Zhao, Haiyan;Shi, Lei;Zhang, Lili;Yu, Lin;Gao, Julin;Yu, Xiaofang;Wang, Zhigang;Shen, Liguo;Ji, Ping;Yang, Shuzong;Zhang, Zhongdong;Xue, Jiquan;Wang, Xiuquan;Wang, Xiuquan;Lu, Tingqi;Dong, Benchun;Li, Gang;Ma, Baoxin;Li, Jinqin;Deng, Xiufeng;Liu, Yonghong;Yang, Qin;Jia, Chunlan;Chen, Xianping;Fu, Hua
关键词：Maize straw production; Straw return; Carbon input; Carbon emission; Soil carbon sequestration
