The Influence of Microwave Sterilization on the Ultrastructure, Permeability of Cell Membrane and Expression of Proteins of Bacillus Cereus
文献类型： 外文期刊
作者： Gao, Jin-Xuan 1 ; Wang, Fang 1 ; Li, Xuan 1 ; Sun, Yang-Ying 1 ; Wang, Ying 1 ; Ou, Chang-Rong 1 ; Shao, Xing-Feng 1 ; Pa 1 ;
作者机构： 1.Ningbo Univ, Key Lab Anim Prot Food Proc Technol Zhejiang Prov, Ningbo, Zhejiang, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Agr Prod Proc, Nanjing, Jiangsu, Peoples R China
关键词： Bacillus cereus; microwave; morphology; the permeability of membrane; proteomics
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）
ISSN： 1664-302X
年卷期： 2018 年 9 卷
页码：
收录情况： SCI
摘要： Bacillus cereus was isolated from ready-to-serve brine goose, identified by 16S rRNA gene sequencing analysis and treated with a commercial microwave sterilization condition (a power of 1,800W at 85 degrees C for 5 min). The influence of microwaves on the morphology, the permeability of membrane and the expression of total bacterial proteins was observed. Microwave induced the clean of bacterial nuclear chromatin, increased the permeability and disrupted the integrity of membrane. Twenty-three proteins including 18 expressed down-regulated proteins and 5 expressed up-regulated proteins were identified by HPLC-MS/MS in the samples treated with microwave. The frequencies of proteins changed after microwaves treatment were labeled as 39.13% (synthesis and metabolism of amino acid or proteins), 21.74% (carbohydrate metabolism), 8.70% (anti-oxidant and acetyl Co-A synthesis), and 4.35% (the catalyst of catabolism of bacterial acetoin, ethanol metabolism, glyoxylate pathway, butyrate synthesis and detoxification activity), respectively. This study indicates that microwaves result in the inactivation of Bacillus cereus by cleaning nuclear chromatin, disrupting cell membrane and disordering the expression of proteins.
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