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Induction of Oxidative DNA Damage in Bovine Herpesvirus 1 Infected Bovine Kidney Cells (MDBK Cells) and Human Tumor Cells (A549 Cells and U2OS Cells)

文献类型： 外文期刊

作者： Zhu, Liqian 1 ; Fu, Xiaotian 1 ; Yuan, Chen 1 ; Jiang, Xinyi 1 ; Zhang, Gaiping 2 ;

作者机构： 1.Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Jiangsu, Peoples R China

2.Jiangsu Co Innovat Ctr Prevent & Control Importan, Yangzhou 225009, Jiangsu, Peoples R China

3.Henan Acad Agr Sci, Key Lab Anim Immunol, Henan Prov Key Lab Anim Immunol, Minist Agr, Zhengzhou 450002, Henan, Peoples R China

4.Henan Agr Univ, Coll Anim Sci & Vet Med, Zhengzhou 450000, Henan, Peoples R China

关键词： BoHV-1; DNA damage; OGG-1; ROS; comet assay; tumor

期刊名称：VIRUSES-BASEL （ 影响因子：5.048； 五年影响因子：5.127 ）

ISSN： 1999-4915

年卷期： 2018 年 10 卷 8 期

页码：

收录情况： SCI

摘要： Bovine herpesvirus 1 (BoHV-1) is an important pathogen of cattle that causes lesions in mucosal surfaces, genital tracts and nervous systems. As a novel oncolytic virus, BoHV-1 infects and kills numerous human tumor cells. However, the mechanisms underlying the virus-induced cell damages are not fully understood. In this study, we demonstrated that virus infection of MDBK cells induced high levels of DNA damage, because the percentage of comet tail DNA (tailDNA%) determined by comet assay, a direct indicator of DNA damage, and the levels of 8-hydroxyguanine (8-oxoG) production, an oxidative DNA damage marker, consistently increased following the virus infection. The expression of 8-oxoguanine DNA glycosylase (OGG-1), an enzyme responsible for the excision of 8-oxoG, was significantly decreased due to the virus infection, which corroborated with the finding that BoHV-1 infection stimulated 8-oxoG production. Furthermore, the virus replication in human tumor cells such as in A549 cells and U2OS cells also induced DNA damage. Chemical inhibition of reactive oxidative species (ROS) production by either ROS scavenger N-Acetyl-l-cysteine or NOX inhibitor diphenylene iodonium (DPI) significantly decreased the levels of tailDNA%, suggesting the involvement of ROS in the virus induced DNA lesions. Collectively, these results indicated that BoHV-1 infection of these cells elicits oxidative DNA damages, providing a perspective in understanding the mechanisms by which the virus induces cell death in both native host cells and human tumor cells.

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