Involvement of a short-type peptidoglycan recognition protein (PGRP) from Chinese giant salamanders Andrias davidianus in the immune response against bacterial infection
文献类型： 外文期刊
作者： Yang, Hui 1 ; Li, Xixi 1 ; Song, Weijia 2 ; Ji, Jiaojun 1 ; Li, Fenggang 3 ; Zhang, Yingying 1 ; Zhang, Xiaojun 1 ; Wang, 1 ;
作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou 225009, Jiangsu, Peoples R China
2.Northwest A&F Univ, Coll Anim Sci & Technol, Yangling 712100, Shaanxi, Peoples R China
3.Chinese Acad Fishery Sci, Yellow River Fisheries Res Inst, Xian 710086, Shaanxi, Peoples R China
关键词： Andrias davidianus; PGRP-SC2; PRR; Immune response
期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.636； 五年影响因子：3.654 ）
ISSN： 0145-305X
年卷期： 2018 年 88 卷
页码：
收录情况： SCI
摘要： PGRPs (Peptidoglycan recognition proteins) could recognize peptidoglycan and play vital roles in innate immunity among different animals. Till present, the functions of PGRP have been studied in various animals, but few reports have studied the amphibian PGRPs. In the current research, a short type PGRP was identified from Chinese giant salamander and its involvement in the innate immunity was studied. The ORF of AdPGRP-SC2 cDNA was 573 bp, which encoded 190 amino acids, and contained a PGRP and an amidase_2 domain. The qPCR analysis revealed that AdPGRP-SC2 mRNA transcripts expressed in different tissues, with the highest expression level in muscle, intestine and spleen. Results of immune challenges with peptidoglycan (PGN) demonstrated that expression patterns of AdPGRP-SC2 were significantly up-regulated in erythrocyte and spleen at the early injection stage. The recombinant AdPGRP-SC2 protein was successfully produced and purified, and it could show binding affinity to different bacteria. In the presence of Zn2+, the rAdPGRP-SC2 could exhibit a broad PAMPs binding activities, strongly agglutinate bacteria and exhibit amidase enzyme activity. Collectively, these data indicate AdPGRP-SC2 could act as PRR to recognize the invading microorganisms and as the antimicrobial effectors during the innate immune response of A. davidianus.
- 相关文献
作者其他论文 更多>>
-
Muti-Biomarker Approach and IBR Index to Evaluate the Oxidative Stress of Nanoplastic on the Clams, Meretrix meretrix and Sinonovacula constricta
作者：Lv, Linlan;Feng, Wanjun;Sun, Ke;Zhao, Weihong;Jiang, Fengjuan;Yang, Yue;Zhang, Yingying;Sui, Yanming;Dong, Xuexing;Mohamed, Mohsen;Mohamed, Mohsen;Li, Lei
关键词：biomarker; antioxidant enzyme;
Meretrix meretrix ;Sinonovacula constricta ; nanoplastics; oxidative stress -
Microplastic Ingestion by Fish with Different Feeding Habits from Different Habitats in a Typical Semi-Enclosed Haizhou Bay
作者：Sui, Yanming;Zhang, Yingying;Chen, Shihao;Shi, Yangyang;Zou, Jiaxin;Qin, Kunyun;Yu, Yebing;Lv, Linlan;Mohamed, Mohsen;Mohamed, Mohsen;Liu, Zhiquan;Sui, Yanming;Zheng, Liang
关键词：microplastics; semi-enclosed bay; feeding habits; feeding modes
-
Female zebrafish ( Danio rerio) ) exposure to polystyrene nanoplastics induces reproductive toxicity in mother and their offspring
作者：Zhang, Chunyun;Yan, Zebang;Wang, Lixin;Xiong, Dongmei;Li, Luoxin;Alava, Juan Jose;Chen, Peng;Gul, Yasmeen
关键词：PS-NPs; Maternal exposure; Reproductive dysfunction; Zebrafish
-
Discovery of an unconventional lamprey lymphocyte lineage highlights divergent features in vertebrate adaptive immune system evolution
作者：Huang, Yingyi;Liu, Xiang;Li, Shuo;Li, Chen;Wang, Hong-Yan;Li, Yanan;Zhang, Xianghui;Wang, Qian;Liu, Kaiqiang;Liu, Yu-Yan;Shao, Changwei;Huang, Yingyi;Liu, Xiang;Li, Shuo;Li, Chen;Wang, Hong-Yan;Li, Yanan;Zhang, Xianghui;Wang, Qian;Liu, Kaiqiang;Liu, Yu-Yan;Shao, Changwei;Liu, Xiang;Liu, Qun;Chen, Jian-Yang;Zhang, Yingying;Fan, Guangyi;Liu, Qun;Chen, Jian-Yang;Zhang, Yingying;Fan, Guangyi;Liu, Qun;Pang, Yue;Liu, Shanshan;Fan, Guangyi;Liu, Shanshan
关键词：
-
Single-cell RNA sequencing illuminates the ontogeny, conservation and diversification of cartilaginous and bony fish lymphocytes
作者：Wang, Hong-Yan;Li, Yanan;Zhang, Xianghui;Liu, Xiang;Lu, Yifang;Li, Yubang;Huang, Yingyi;Li, Shuo;Zhang, Yangqing;Han, Shenglei;Jiang, Shuhong;Wang, Rui;Liu, Yuyan;Liu, Kaiqiang;Wang, Qian;Shao, Changwei;Wang, Hong-Yan;Li, Yanan;Zhang, Xianghui;Liu, Xiang;Lu, Yifang;Li, Yubang;Huang, Yingyi;Li, Shuo;Zhang, Yangqing;Han, Shenglei;Jiang, Shuhong;Wang, Rui;Liu, Yuyan;Liu, Kaiqiang;Wang, Qian;Shao, Changwei;Chen, Jian-Yang;He, Hang;Chen, Hongxi;Liu, Qun;Yang, Mingming;Zhang, Yingying;Zhou, Li;Tan, Fujian;Meng, Liang;Fan, Guangyi;Chen, Jian-Yang;He, Hang;Chen, Hongxi;Liu, Qun;Yang, Mingming;Zhang, Yingying;Zhou, Li;Tan, Fujian;Meng, Liang;Fan, Guangyi;Li, Yanan;Liu, Xiang;Jia, Zhao;Zou, Jun;Ji, Qianyue;Liu, Shanshan;Seim, Inge;Seim, Inge;Fan, Guangyi
关键词：
-
Comparative Genomic Analysis Unveils Potential Factors Contributing to the Endangerment of Silurus lanzhouensis
作者：Yang, Yuanhao;Zhang, Yameng;Li, Fenggang;Wen, Sien;Wang, Lvzhou;Lan, Guozhu;Yang, Yuanhao;Zhang, Yameng;Li, Fenggang;Wen, Sien;Wang, Lvzhou;Lan, Guozhu;Zhou, Jishu;Li, Yang;Zhu, Zilin
关键词：Silurus lanzhouensis; genome; chromosomal assembly; comparative genomic analysis
-
Triazophos exposure on maternal Daphnia magna at environmental-related concentrations revealed toxic effects to its offspring
作者：Liu, Shaoquan;Zhang, Chunyun;Wang, Lixin;Liang, Hao;Xiong, Dongmei;Zhang, Jianlu;Li, Ruijiao;Feng, Guangpeng
关键词：Triazophos; Daphnia magna; Chronic exposure; Reproduction effect; Environmental-related concentration
