文献类型： 外文期刊
作者： Wang, Yunpeng 1 ; Wei, Zhengyi 1 ; Xing, Shaochen 1 ;
作者机构： 1.Jilin Acad Agr Sci, Inst Agr Biotechnol, Changchun 130033, Jilin, Peoples R China
关键词： Rice; Plastid transformation; Green fluorescent protein; Homoplastomic selection
期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）
ISSN： 0006-291X
年卷期： 2018 年 503 卷 4 期
页码：
收录情况： SCI
摘要： Rice is one of the most important cereal crops and its biotechnology has been pursued to meet the food demand of ever-growing global population. Rice plastid transformation has been a great challenge to achieve homoplastomic plants due to its low efficiency of regeneration. In this experiment, Japonica rice line 19 was chosen to be the receptor for plastid transformation. A vector harboring smGFP gene was constructed and transferred into rice plastid genome by bombardment. The resistant callus was obtained after long-lasting multiple selections and proved to be in homoplastomic status by molecular testing. The plantlet was regenerated from homoplastomic callus and grown to seeding stage. This is the first case so far to achieve the homoplastomic rice and will be helpful to transform plastid genome of monocotyledonous crops with recalcitrant nature. (C) 2018 Elsevier Inc. All rights reserved.
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